Determination of erythromycin A by liquid chromatography and electrochemical detection, with application to salmon tissue
| Autor: | Mojmir Janeček, David H. North, M. Ruth Bailey, Michael A. Quilliam |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Aqueous solution
Chromatography Meat Tertiary amine Metabolite Sodium Erythromycin chemistry.chemical_element General Chemistry Reversed-phase chromatography Electrochemical detection Trace Elements chemistry.chemical_compound chemistry Salmon medicine Electrochemistry Animals Quantitative analysis (chemistry) medicine.drug Chromatography Liquid |
| Zdroj: | Journal of chromatography. 619(1) |
| Popis: | The chromatographic performance of erythromycin A (EA) is improved significantly over that achieved on polymeric columns by using a sterically shielded octyldiisopropylsilica (Zorbax Rx-C8) column and a neutral mobile phase consisting of 5 mM aqueous sodium perchlorate—acetonitrile (50:50). This mobile phase facilitates electrochemical detection of EA at the 3-pmol level. Temperature control of both column and detection cell is important for minimizing detector noise and drift. A clean-up procedure, based on aminopropylsilica solid-phase extraction, allows the detection of EA in salmon flesh down to the 0.2-ppm level. Some of the metabolites of EA that retain the tertiary amine may also be detected by this method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|