Assembly of JC virus-like particles in COS7 cells

Autor:	Shigeru Morikawa, Jun Mukaigawa, Kotaro Yasui, Soichi Nukuzuma, Yukiko Shishido, Kazuo Nagashima
Rok vydání:	1997
Předmět:	Cell Nucleus COS cells Virus Assembly viruses Virion JC virus Transfection Biology medicine.disease_cause JC Virus Virology Virus Capsid Infectious Diseases Viral replication Virus-like particle Cell culture COS Cells medicine Animals Humans Capsid Proteins
Zdroj:	Journal of Medical Virology. 51:265-272
ISSN:	1096-9071 0146-6615
DOI:	10.1002/(sici)1096-9071(199704)51:4<265::aid-jmv2>3.0.co;2-3
Popis:	JC virus lacks an appropriate cell line to support virus replication. The establishment of a JC pseudovirus assembly system could play an alternative role for a virus culture system. COS7 cells and a transfer vector, pcDL-SR alpha 296, were used to express JC viral structural genes. VP231-SR alpha, which encodes VP2/VP3 and VP1, but lacks 137 bp of the 5'-terminus of agnogene, showed both efficient nuclear migration and quantitative expression of the major capsid protein VP1. JC pseudovirus assembly was observed in the nucleus of VP231-SR alpha transfected cells. Evidence of JC pseudovirus assembly is presented. The further utilization of this system, which includes a study for the viral morphogenesis, serological diagnosis, as well as the potential application for gene transfer vector, is discussed.
Databáze:	OpenAIRE
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