Dominant antibody responses to Fucα1-3GalNAc and Fucα1-2Fucα1-3GlcNAc containing carbohydrate epitopes in Pan troglodytes vaccinated and infected with Schistosoma mansoni
| Autor: | Johannis P. Kamerling, I. van Die, R. A. Wilson, D. H. Van Den Eijnden, A.M. van Roon, Károly Ágoston, Johannes F.G. Vliegenthart, G.J. van Dam, A. van Remoortere, János Kerékgyártó, H. J. Vermeer, J.A. Langermans, Cornelis H. Hokke, Alan W. Thomas, André M. Deelder |
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| Přispěvatelé: | Chemie van glyco-conjugaten, Dep Scheikunde |
| Rok vydání: | 2006 |
| Předmět: |
Male
Pan troglodytes Molecular Sequence Data Immunology Antibodies Helminth Disaccharides Immunoglobulin G Epitope Epitopes Immune system Polysaccharides Animals Parasite hosting Longitudinal Studies biology Spectrum Analysis Vaccination Schistosoma mansoni General Medicine biology.organism_classification Virology Schistosomiasis mansoni Infectious Diseases Carbohydrate Sequence Immunoglobulin M biology.protein Parasitology Trematoda Antibody Trisaccharides |
| Popis: | The development of the humoral anti-glycan immune response of chimpanzees, either or not vaccinated with radiation-attenuated Schistosoma mansoni cercariae, was followed during 1 year after infection with S. mansoni. During the acute phase of infection both the vaccinated and the control chimpanzees produce high levels of immunoglobulin G (IgG) antibodies against carbohydrate structures that are characteristic for schistosomes carrying the Fucalpha1-3GalNAc and Fucalpha1-2Fucalpha1-3GlcNAc motifs, but not to the more widespread occurring structures GalNAcbeta1-4GlcNAc, GalNAcbeta1-4(Fucalpha1-3)GlcNAc, and Galbeta1-4(Fucalpha1-3)GlcNAc (Lewis(x)). In addition, high levels of IgM antibodies were found against the trimeric Lewis(x) epitope. Apparently, the schistosome-characteristic carbohydrate structures are dominant epitopes in the anti-glycan humoral immune response of the chimpanzees. All chimpanzees showed an increase in the level of antibodies against most of the carbohydrate structures tested directly after vaccination, peaking at challenge time and during the acute phase of infection. With the exception of anti-F-LDN antibody responses, the anti-carbohydrate antibody responses upon schistosome infection of the vaccinated animals were muted in comparison to the control animals. |
| Databáze: | OpenAIRE |
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