Increased dietary calcium inclusion in fully acidified prepartum diets improved postpartum uterine health and fertility when fed to Holstein cows

Autor: Kelly T. Ryan, Andrew J. Steelman, A.D. Rowson, Felipe C. Cardoso, S.S. Bascom, K.M. Glosson, A.R. Guadagnin
Rok vydání: 2020
Předmět:
medicine.medical_treatment
media_common.quotation_subject
Uterus
Cattle Diseases
Ice calving
03 medical and health sciences
0302 clinical medicine
Animal science
Food Animals
Pregnancy
Blood plasma
medicine
Animals
Lactation
Small Animals
Ovulation
media_common
chemistry.chemical_classification
030219 obstetrics & reproductive medicine
biology
Equine
Artificial insemination
Glutathione peroxidase
Postpartum Period
Haptoglobin
0402 animal and dairy science
Maternal Nutritional Physiological Phenomena
Puerperal Disorders
04 agricultural and veterinary sciences
Animal Feed
040201 dairy & animal science
Diet
Calcium
Dietary
Fertility
Milk
medicine.anatomical_structure
chemistry
biology.protein
Animal Nutritional Physiological Phenomena
Cattle
Female
Animal Science and Zoology
Uterine gland
Endometritis
Acids
Zdroj: Theriogenology. 142:338-347
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2019.10.014
Popis: The objective of this study was to determine the effects of prepartum negative dietary cation-anion difference diet (DCAD) fed at two dietary Ca inclusion rates on postpartum uterine health and ovulation dynamics of multiparous Holstein cows (n = 76). Treatments began at 28 days before expected calving until parturition and were: CON: DCAD = +6 mEq/100g of DM with low dietary Ca (46.2 ± 15.2 g Ca/d; 0.4% DM; n = 26); ND: DCAD = -24 mEq/100g of DM with low dietary Ca (44.1 ± 16.1 Ca/d; 0.4% DM; n = 24); NDCA: DCAD = -24 mEq/100g of DM with high dietary Ca (226.6 ± 96.0 g Ca/d; 2.0% DM; n = 26). Vaginal discharge was evaluated through the fresh period via Metricheck (MC) for presence of purulent material. Polymorphonuclear (PMN) cell concentration in the uterus was evaluated at 15 and 30 days relative to calving (DRC). Endometrial tissue was harvested at 30 DRC for glandular morphology, presence of tight-junctions and adheren-junctions proteins, as well as assessment of superoxide dismutase (SOD) and glutathione peroxidase (GPX) activity. Blood plasma and serum samples were harvested in the prepartum and postpartum phase and were assessed for concentrations of lipopolysaccharide binding protein (LBP), serum amyloid A (SAA), and haptoglobin (HP). Ovarian dynamics were assessed through the fresh period until first timed artificial insemination (TAI). Cows fed CON had a lower MC score (P = 0.06) than the average of cows fed ND and cows fed NDCA. Cows fed ND had a higher MC score than cows fed NDCA. Cows fed NDCA had greater uterine gland epithelial height (P = 0.02) than cows fed ND. Cows fed NDCA also had a greater number of epithelial cells per gland (P = 0.05) than cows fed ND. Cows fed NDCA had greater intensity of occludin expression (P = 0.15) than cows fed ND. Cows fed NDCA had increased activity of SOD (P = 0.05) and decreased activity of GPX (P 0.001) than cows fed ND. Cows fed ND had higher plasma HP concentrations than cows fed NDCA in the prepartum (P = 0.01) and post-partum (P = 0.03) periods. Cows fed ND and NDCA had lower (P = 0.01) postpartum plasma HP concentration than cows fed CON. In conclusion, cows fed NDCA had an improved uterine environment most likely due to alleviation of oxidative stress, an enhanced immune response to parturition and uterine discharge comparable to cows fed CON.
Databáze: OpenAIRE
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