Biological profile of monocyte-derived macrophages in coronary heart disease patients: implications for plaque morphology

Autor: Francesco Fracassi, Nicola Cosentino, Giampaolo Niccoli, Alice Bonomi, Hesham Refaat, Giuseppe Calligaris, Filippo Crea, Fabrizio Veglia, Susanna Fiorelli, Sonia Eligini, Franco Fabbiocchi, Giancarlo Marenzi, Marina Camera, Elena Tremoli, Stefano De Martini
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
Cell biology
Pathology
medicine.medical_specialty
Antigens
Differentiation
Myelomonocytic
Lumen (anatomy)
lcsh:Medicine
Receptors
Cell Surface
Diseases
Coronary Artery Disease
Article
Thromboplastin
Coronary artery disease
03 medical and health sciences
Tissue factor
0302 clinical medicine
Antigen
Antigens
CD
GTP-Binding Proteins
In vivo
Humans
Medicine
Lectins
C-Type
Protein Glutamine gamma Glutamyltransferase 2
Receptor
Efferocytosis
lcsh:Science
Cell Shape
Cells
Cultured
Aged
Transglutaminases
Multidisciplinary
business.industry
Macrophages
lcsh:R
Middle Aged
Atherosclerosis
medicine.disease
Plaque
Atherosclerotic
Mannose-Binding Lectins
030104 developmental biology
Female
lcsh:Q
business
CD163
Mannose Receptor
Tomography
Optical Coherence
030217 neurology & neurosurgery
Zdroj: Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
Scientific Reports
ISSN: 2045-2322
DOI: 10.1038/s41598-019-44847-3
Popis: The prevalence of a macrophage phenotype in atherosclerotic plaque may drive its progression and/or instability. Macrophages from coronary plaques are not available, and monocyte-derived macrophages (MDMs) are usually considered as a surrogate. We compared the MDM profile obtained from coronary artery disease (CAD) patients and healthy subjects, and we evaluated the association between CAD MDM profile and in vivo coronary plaque characteristics assessed by optical coherence tomography (OCT). At morphological analysis, MDMs of CAD patients had a higher prevalence of round than spindle cells, whereas in healthy subjects the prevalence of the two morphotypes was similar. Compared to healthy subjects, MDMs of CAD patients had reduced efferocytosis, lower transglutaminase-2, CD206 and CD163 receptor levels, and higher tissue factor (TF) levels. At OCT, patients with a higher prevalence of round MDMs showed more frequently a lipid-rich plaque, a thin-cap fibroatheroma, a greater intra-plaque macrophage accumulation, and a ruptured plaque. The MDM efferocytosis correlated with minimal lumen area, and TF levels in MDMs correlated with the presence of ruptured plaque. MDMs obtained from CAD patients are characterized by a morpho-phenotypic heterogeneity with a prevalence of round cells, showing pro-inflammatory and pro-thrombotic properties. The MDM profile allows identifying CAD patients at high risk.
Databáze: OpenAIRE
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