Determination of Methemoglobin in Hemoglobin Submicron Particles Using NMR Relaxometry
| Autor: | Kaewprayoon, Waraporn, Suwannasom, Nittiya, Kloypan, Chiraphat, Steffen, Axel, Xiong, Yu, Schellenberger, Eyk, Pruß, Axel, Georgieva, Radostina, Bäumler, Hans |
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| Rok vydání: | 2020 |
| Předmět: |
Erythrocytes
Magnetic Resonance Spectroscopy hemoglobin microparticles (Hb-MP) Serum Albumin Human Article hemoglobin-based oxygen carriers 1H2O NMR-relaxometry Solutions lcsh:Chemistry Hemoglobins Cross-Linking Reagents lcsh:Biology (General) lcsh:QD1-999 Glutaral Humans lcsh:QH301-705.5 methemoglobin 600 Technik Medizin angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit |
| Zdroj: | International Journal of Molecular Sciences Volume 21 Issue 23 International Journal of Molecular Sciences, Vol 21, Iss 8978, p 8978 (2020) |
| DOI: | 10.17169/refubium-28755 |
| Popis: | Methemoglobin (MetHb) is a hemoglobin (Hb) derivative with the heme iron in ferric state (Fe3+), unable to deliver oxygen. Quantification of methemoglobin is a very important diagnostic parameter in hypoxia. Recently, novel hemoglobin microparticles (Hb-MP) with a narrow size distribution around 700 nm, consisting of cross-linked Hb were proposed as artificial oxygen carriers. The cross-linking of Hb by glutaraldehyde (GA) generates a certain amount of MetHb. Due to the strong light scattering, quantitative determination of MetHb in Hb-MP suspensions by common spectrophotometry is not possible. Here, we demonstrate that 1H2O NMR relaxometry is a perfect tool for direct measurement of total Hb and MetHb concentrations in Hb-MP samples. The longitudinal relaxation rate 1/T1 shows a linear increase with increasing MetHb concentration, whereas the transverse relaxation rate 1/T2 linearly increases with the total Hb concentration. In both linear regressions the determination coefficient (R2) is higher than 0.99. The method does not require time-consuming pretreatment or digestion of the particles and is not impaired by light scattering. Therefore, it can be established as the method of choice for the quality control of Hb-MP and similar hemoglobin-based oxygen carriers in the future. |
| Databáze: | OpenAIRE |
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