Continuous Online Protein Quality Monitoring during Perfusion Culture Production Using an Integrated Micro/Nanofluidic System
| Autor: | Jean-François P. Hamel, Sung Hee Ko, Taehong Kwon, Jongyoon Han |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microfluidics
CHO Cells 010402 general chemistry 01 natural sciences Article Analytical Chemistry Perfusion Culture Cricetulus Lab-On-A-Chip Devices Animals Nanotechnology Biomanufacturing Process engineering Manufacturing efficiency Cells Cultured Protein size business.industry Chemistry 010401 analytical chemistry Continuous monitoring Proteins Perfusion bioreactor 0104 chemical sciences Perfusion business Protein quality |
| Zdroj: | Analytical Chemistry |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | We demonstrate a new micro/nanofluidic system for continuous and automatic monitoring of protein product size and quantity directly from the culture supernatant during a high-cell-concentration CHO cell perfusion culture. A microfluidic device enables clog-free cell retention for a bench-scale (350 mL) perfusion bioreactor that continuously produces the culture supernatant containing monoclonal antibodies (IgG1). A nanofluidic device directly monitors the protein size and quantity in the culture supernatant. The continuous-flow and fully automated operation of this nanofluidic protein analytics reduces design complexity and offers more detailed information on protein products than offline and batch-mode conventional analytics. Moreover, chemical and mechanical robustness of the nanofluidic device enables continuous monitoring for several days to a week. This continuous and online protein quality monitoring could be deployed at different steps and scales of biomanufacturing to improve product quality and manufacturing efficiency. |
| Databáze: | OpenAIRE |
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