Generation of poly-β-hydroxybutyrate from acetate in higher plants: Detection of acetoacetyl CoA reductase- and PHB synthase- activities in rice

Autor: Terumi Saito, Eri Inoue, Hirohisa Tsuda, Mari Shiraki
Rok vydání: 2016
Předmět:
0106 biological sciences
0301 basic medicine
Physiology
Polyesters
030106 microbiology
Colony Count
Microbial
Hydroxybutyrates
Alcohol oxidoreductase
Ethylenediaminetetraacetic acid
macromolecular substances
Plant Science
Acetates
Zea mays
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Ralstonia
Carbon Radioisotopes
chemistry.chemical_classification
Rhizosphere
biology
Acetoacetyl-CoA reductase
Chemistry
Acetyl-CoA
technology
industry
and agriculture
food and beverages
Oryza
biology.organism_classification
Absorption
Physiological
Alcohol Oxidoreductases
Enzyme
Biochemistry
Seedlings
Crassulacean acid metabolism
lipids (amino acids
peptides
and proteins)
Agronomy and Crop Science
Acyltransferases
Metabolic Networks and Pathways
Subcellular Fractions
010606 plant biology & botany
Zdroj: Journal of Plant Physiology. 201:9-16
ISSN: 0176-1617
DOI: 10.1016/j.jplph.2016.06.007
Popis: It has been reported that Poly-β-hydroxybutyrate (PHB) is generated from acetate in the rice root. However, no information is available about the biosynthetic pathway of PHB from acetate in plant cells. In the bacterium Ralstonia eutropha H16 (R. eutropha), PHB is synthesized from acetyl CoA by the consecutive reaction of three enzymes: β-ketothiolase (EC: 2.3.1.9), acetoacetyl CoA reductase (EC: 1.1.1.36) and PHB synthase (EC: 2.3.1.-). Thus, in this study, we examined whether the above three enzymatic activities were also detected in rice seedlings. The results clearly showed that the activities of the above three enzymes were all detected in rice. In particular, the PHB synthase activity was detected specifically in the sonicated particulate fractions (2000g 10min precipitate (ppt) and the 8000g 30min ppt) of rice roots and leaves. In addition to these enzyme activities, several new experimental results were obtained on PHB synthesis in higher plants: (a) (14)C-PHB generated from 2-(14)C-acetate was mainly localized in the 2000g 10min ppt and the 8000g 30min ppt of rice root. (b) Addition of acetate (0.1-10mM) to culture medium of rice seedlings did not increase the content of PHB in the rice root or leaf. (c) In addition to C3 plants, PHB was generated from acetate in a C4 plant (corn) and in a CAM plant (Bryophyllum pinnatum). d) Washing with ethylenediaminetetraacetic acid (EDTA) strongly suggested that the PHB synthesized from acetate was of plant origin and was not bacterial contamination.
Databáze: OpenAIRE
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