Baicalin attenuates myocardial ischemia-reperfusion injury through Akt/NF-κB pathway
| Autor: | Qian Xin, Jue Wang, Chao Sun, Yi-Biao Wang, Yun Luan, Zhaohua Zhang, Wen Jiang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Ischemia Inflammation Myocardial Reperfusion Injury Pharmacology Biochemistry 03 medical and health sciences chemistry.chemical_compound Phosphatidylinositol 3-Kinases 0302 clinical medicine Western blot medicine Animals Molecular Biology Protein kinase B PI3K/AKT/mTOR pathway bcl-2-Associated X Protein Flavonoids medicine.diagnostic_test business.industry Caspase 3 Interleukin-6 Tumor Necrosis Factor-alpha Interleukin-8 NF-kappa B Cell Biology medicine.disease Interleukin-10 Rats 030104 developmental biology chemistry Apoptosis Echocardiography 030220 oncology & carcinogenesis medicine.symptom business Reperfusion injury Baicalin Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | Journal of cellular biochemistry. 120(3) |
| ISSN: | 1097-4644 |
| Popis: | Background Baicalin can attenuate myocardial ischemia-reperfusion (I/R) on damage. However, the mechanisms are still not fully understood. The study aimed to investigate the antiapoptosis and anti-inflammatory effects of baicalin on myocardial I/R-induced injury. Methods We established male rats I/R model, and baicalin was intragastric administration after ischemia onset. All experimental animals were randomly divided into five groups: group I, sham; group II, I/R; group III, 50 mg/kg; group IV, 100 mg/kg; and group V, 200 mg/kg baicalin. Postoperation, left ventricular (LV) function was recorded by transthoracic echocardiography. Myocardial infarct size, number of vessels and apoptosis were detected by histology and immunohistochemistry. Furthermore, the messenger RNA (mRNA) and protein levels of tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), IL-6, IL-8, IL-10, Bcl2, Bax, caspase-3, phosphatidylinositol 3-kinase (PI3K), Akt, p-Akt, and nuclear factor-κB (NF-κB) p65 in myocardial tissues were measured by quantitative real-time polymerase chain reaction and Western blot analysis assays. Result When compared with I/R groups, baicalin could significantly improve LV hemodynamic parameters. Myocardial infarct size and apoptosis were significantly decreased, but the vessel density was increased. The mRNA levels of TNF-α, IL-1β, IL-6, and IL-8 were downregulated, but the levels of IL-10, proapoptotic genes caspase-3, and the ratio of Bax/Bcl2 were upregulated. Moreover, the protein expression of PI3K, p-Akt, and Akt were upregulated but NF-κB p65 was downregulated in the groups III, IV, and V than in group II. Conclusion Our current study suggested that baicalin attenuated myocardial I/R-induced damage, inhibited myocardial apoptosis, and inflammation by activating PI3K/Akt but suppressing NF-κB signaling. |
| Databáze: | OpenAIRE |
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