Evaluation of cytotoxicity of hematite nanoparticles in bacteria and human cell lines
| Autor: | Kumar Rajendran, T. Vinoth Kumar, Shampa Sen, G. Suja, S. Lakshmana Senthil |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Erythrocytes Lysis Cell Survival 030106 microbiology Bacillus cereus Metal Nanoparticles Nanoparticle 010501 environmental sciences Bacterial growth Ferric Compounds Hemolysis 01 natural sciences Cell Line Microbiology 03 medical and health sciences Colloid and Surface Chemistry Chlorocebus aethiops Animals Humans Response surface methodology Particle Size Physical and Theoretical Chemistry Cytotoxicity Vero Cells 0105 earth and related environmental sciences biology Chemistry Surfaces and Interfaces General Medicine Hematite biology.organism_classification Anti-Bacterial Agents Chemical engineering A549 Cells visual_art MCF-7 Cells visual_art.visual_art_medium Bacteria Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 157:101-109 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/j.colsurfb.2017.05.052 |
| Popis: | Metal nanoparticles have a great impact and even change the composition of soil microbial communities. This poses the risk of their accumulation in the ecosystem, which may call on health hazard. Statistical techniques such as Plackett-Burman design, Response Surface Methodology were used for optimizing medium constituents for Bacillus cereus SVK1 and other critical variables responsible for the production of biomolecules and biosynthesis of hematite nanoparticles. The effect of hematite nanoparticles on the growth of soil bacteria were tested by agar-well diffusion method and dynamic growth curve techniques. Bacterial growth inhibition was not observed with hematite nanoparticle concentration of up to 25mg/mL. In addition, hematite nanoparticles enhanced the growth of the soil bacteria. The results show that hematite nanoparticles were non-toxic to soil bacteria indicating their scope for wide range of applications. The effective cytotoxic concentration (CTC50) of hematite nanoparticles against MCF-7, A549, Hep3B and Vero cell lines was found to be 207.58, 224.69, 193.26 and 2530μg/mL respectively. Hematite nanoparticles didn't cause lysis of red blood cells. |
| Databáze: | OpenAIRE |
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