Development and characterization of eight polymorphic microsatellite markers forDaphnia atkinsoni(Crustacea: Ctenodaphnia)
Autor: | J. K. J. Van Houdt, Joachim Mergeay, Raquel Ortells, L. De Meester, Sarah Geldof |
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Rok vydání: | 2009 |
Předmět: | |
Zdroj: | Molecular Ecology Resources. 9:326-329 |
ISSN: | 1755-0998 1755-098X |
Popis: | A microsatellite-enriched genomic library was developed for the water flea Daphnia atkinsoni Baird, 1859, a dominant species of intermittent wetlands in Europe. Eight polymorphic microsatellite markers were successfully optimized. Characterization of 77 individuals from Belgium and Spain showed moderate (in the former) to high (in the latter) levels of polymorphism with two to 11 alleles per locus and an observed heterozygosity ranging from 0 to 0.87. Some of these microsatellite markers were successfully amplified in three other Daphnia species (D. magna n = 4, D. similis n = 6; D. obtusa n = 6). |
Databáze: | OpenAIRE |
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