Structural basis of astrocytic Ca2+ signals at tripartite synapses
| Autor: | Luigi Bellocchio, Jillian L. Stobart, U. Valentin Nägerl, Katsuhiko Mikoshiba, Aude Panatier, Stéphane H. R. Oliet, V. V. G. Krishna Inavalli, Mirelle J. T. Ter Veer, Thomas Pfeiffer, Florian Levet, Giovanni Marsicano, Misa Arizono, Bruno Weber, Julie Angibaud |
|---|---|
| Přispěvatelé: | Université de Bordeaux (UB), Interdisciplinary Institute for Neuroscience [Bordeaux] (IINS), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), NeuroCentre Magendie [Bordeaux], Institut National de la Santé et de la Recherche Médicale (INSERM), Bordeaux Imaging Center (BIC), Université de Bordeaux (UB)-Institut François Magendie-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Universität Zürich [Zürich] = University of Zurich (UZH), ShanghaiTech University [Shanghai], The study was supported by research grants from the Agence Nationale de la Recherche (ANR-12-BSV4-0014 and ANR-17-CE16-0002 to UVN and SHRO, ANR-16-CE16-0001-0 to A.P.) and Fondation pour la Recherche Médicale (DEQ20160334901) and LabEx BRAIN. M.A. was supported by postdoctoral fellowships from RIKEN and JSPS (Japan)., ANR-12-BSV4-0014,SUPERtri,Imagerie super-résolutive de la synapse tripartite(2012), ANR-17-CE16-0002,CASTRO,Contribution des récepteurs IP3 et du réticulum endoplasmique à la signalisation Ca2+ dans les astrocytes(2017), ANR-16-CE16-0001,ASTRODOM,Exploration fonctionnelle du domaine astrocytaire(2016), University of Zurich, Nägerl, U Valentin, Bodescot, Myriam, BLANC - Imagerie super-résolutive de la synapse tripartite - - SUPERtri2012 - ANR-12-BSV4-0014 - BLANC - VALID, Contribution des récepteurs IP3 et du réticulum endoplasmique à la signalisation Ca2+ dans les astrocytes - - CASTRO2017 - ANR-17-CE16-0002 - AAPG2017 - VALID, Exploration fonctionnelle du domaine astrocytaire - - ASTRODOM2016 - ANR-16-CE16-0001 - AAPG2016 - VALID |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Dendritic spine Science [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology Hippocampus 10050 Institute of Pharmacology and Toxicology General Physics and Astronomy 610 Medicine & health 1600 General Chemistry Genetics and Molecular Biology Hippocampal formation General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine 1300 General Biochemistry Genetics and Molecular Biology lcsh:Science Multidisciplinary Chemistry Endoplasmic reticulum [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology STED microscopy General Chemistry Barrel cortex 3100 General Physics and Astronomy 030104 developmental biology Reticular connective tissue General Biochemistry 570 Life sciences biology lcsh:Q Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Nature Communications Nature Communications, Nature Publishing Group, 2020, 11 (1), pp.1906. ⟨10.1038/s41467-020-15648-4⟩ Nature Communications, Vol 11, Iss 1, Pp 1-15 (2020) Nature Communications, 2020, 11 (1), pp.1906. ⟨10.1038/s41467-020-15648-4⟩ |
| ISSN: | 2041-1723 |
| Popis: | Astrocytic Ca2+ signals can be fast and local, supporting the idea that astrocytes have the ability to regulate single synapses. However, the anatomical basis of such specific signaling remains unclear, owing to difficulties in resolving the spongiform domain of astrocytes where most tripartite synapses are located. Using 3D-STED microscopy in living organotypic brain slices, we imaged the spongiform domain of astrocytes and observed a reticular meshwork of nodes and shafts that often formed loop-like structures. These anatomical features were also observed in acute hippocampal slices and in barrel cortex in vivo. The majority of dendritic spines were contacted by nodes and their sizes were correlated. FRAP experiments and Ca2+ imaging showed that nodes were biochemical compartments and Ca2+ microdomains. Mapping astrocytic Ca2+ signals onto STED images of nodes and dendritic spines showed they were associated with individual synapses. Here, we report on the nanoscale organization of astrocytes, identifying nodes as a functional astrocytic component of tripartite synapses that may enable synapse-specific communication between neurons and astrocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|