Isolation and Identification of Yeasts from Wild Flowers Collected around Jangseong Lake in Jeollanam-do, Republic of Korea, and Characterization of the Unrecorded Yeast Bullera coprosmaensis
| Autor: | Se-Hee Hyun, Ha-Kun Kim, Jong-Soo Lee, Hyang Burm Lee, Sang-Min Han, Hye Won Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Bullera coprosmaensis business.industry Basic Local Alignment Search Tool Pseudozyma rugulosa 030108 mycology & parasitology Biology Yeast strain Isolation (microbiology) Microbiology Yeast Biotechnology Unrecorded yeast 03 medical and health sciences 030104 developmental biology Infectious Diseases Characteristics Botany Jangseong Lake Halotolerance business Ribosomal DNA Wild flowers Research Article |
| Zdroj: | Mycobiology |
| ISSN: | 2092-9323 1229-8093 |
| Popis: | Several types of yeasts were isolated from wild flowers around Jangseong Lake in Jeollanam-do, Republic of Korea and identified by comparing the nucleotide sequences of the PCR amplicons for the D1/D2 variable domain of the 26S ribosomal DNA using Basic Local Alignment Search Tool (BLAST) analysis. In total, 60 strains from 18 species were isolated, and Pseudozyma spp. (27 strains), which included Pseudozyma rugulosa (7 strains) and Pseudozyma aphidis (6 strains), was dominant species. Among the 60 strains, Bullera coprosmaensis JS00600 represented a newly recorded yeast strain in Korea, and its microbiological characteristics were investigated. The yeast cell has an oval-shaped morphology measuring 1.4 × 1.7 µm in size. Bullera coprosmaensis JS00600 is an asporous yeast that exhibits no pseudomycelium formation. It grew well in vitamin-free medium as well as in yeast extract-malt extract broth and yeast extract-peptone-dextrose (YPD) broth, and it is halotolerant growing in 10% NaCl-containing YPD broth. |
| Databáze: | OpenAIRE |
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