| Autor: |
Amy B. Heimberger, George A. Calin, Gregory N. Fuller, Ganesh Rao, Frederick F. Lang, Nicholas B. Levine, Wei Qiao, Olsi Gjyshi, Krishan Jethwa, Kayla McEnery, Yuhui Yang, Sherise D. Ferguson, Tiffany Doucette, Shuo Xu, Ling-Yuan Kong, Fei Wang, Jun Wei |
| Rok vydání: |
2023 |
| DOI: |
10.1158/0008-5472.22394871.v1 |
| Popis: |
Supplementary Figure 5 - PDF file 77K, Up regulation of miR-124 level and down regulation of p-STAT3 in peripheral blood T-cells and glioma- infiltrating T-cells after intravenous administration of miR- 124. (A) The miR-124 expression level is below the limit of detection in CD3+ T-cells isolated from the blood of non-tumor bearing C57/BL6J mice (n = 3) and GL261-bearing mice (n = 3) ( P > 0.05). Levels are shown relative to U6 snRNA. (B) miR-124 is detected in both CD3+ T-cells isolated from blood ( P < 0.05 relative to scramble control; n=3) and gliomas ( P < 0.05 relative to scramble control; n=3) 18 hours after the in vivo administration of miR-124. (C) Coinciding with the presence of miR-124, p-STAT3 expression levels were decreased in the T-cells from the peripheral blood and gliomas in the miR-124 treated mice. Representative histograms were shown. The p-STAT3 expression levels relative to isotype control in the peripheral blood T-cells in the miR-124 treated mice (1.4 � 0.2%) was down-modulated compared to scramble treated mice (17.9 � 1.9%) ( P = 0.007; n=3) and glioma-infiltrating T-cells (miR-124: 4.3 � 0.1%; scramble: 18.2 � 4.3%; P = 0.007), but not within the splenic T-cells (miR-124: 16.8 � 0.9%; scramble: 12.9 � 2.7%; P = 0.07) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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