LncRNA MEG3 alleviates PFOS induced placental cell growth inhibition through its derived miR-770 targeting PTX3
| Autor: | Jia-Wei Hong, Zhenyao Huang, Gang Chen, Xinxin Yang, Xiaojie Quan, Saifei Lei, Qi Wang, Jing Li, Weiyi Song |
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| Rok vydání: | 2021 |
| Předmět: |
Health
Toxicology and Mutagenesis Placenta Nerve Tissue Proteins Toxicology Andrology chemistry.chemical_compound Mice Pregnancy medicine Animals MEG3 Fetus Fluorocarbons Cell growth Chemistry General Medicine PTX3 Pollution MicroRNAs medicine.anatomical_structure C-Reactive Protein Alkanesulfonic Acids DNA methylation Toxicity Female RNA Long Noncoding Growth inhibition |
| Zdroj: | Environmental pollution (Barking, Essex : 1987). 293 |
| ISSN: | 1873-6424 |
| Popis: | Perfluorooctane sulfonic acid (PFOS) is a persistent environmental pollutant. Exposure to PFOS has been associated with abnormal fetal development. The long non-coding RNA (lncRNA) has been showed to play a role in fetal growth restriction (FGR), preeclampsia (PE) and other pregnancy complications. Whether the lncRNA contributes to PFOS-induced toxicity in the placenta remains unknown. In this study, we investigated the function of lncRNA MEG3 and its derived miR-770 in PFOS-induced placental toxicity. Pregnant mice received gavage administration of different concentrations of PFOS (0.5, 2.5, and 12.5 mg/kg/day) from GD0 to GD17, and HTR-8/SVneo cells were treated with PFOS in the concentrations of 0, 10−1, 1, 10 μM. We found that expression levels of miR-770 and its host gene MEG3 were reduced in mice placentas and HTR-8/SVneo cells with exposure of PFOS. A significant hypermethylation was observed at MEG3 promoter in placentas of mice gestational-treated with PFOS. We also confirmed that MEG3 and miR-770 overexpression alleviated the cell growth inhibition induced by PFOS. Furthermore, PTX3 (Pentraxin 3) was identified as the direct target of miR-770 and it was enhanced after PFOS exposure. In summary, our results suggested that MEG3 alleviate PFOS-induced placental cell inhibition through MEG3/miR-770/PTX3 axis. |
| Databáze: | OpenAIRE |
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