Herpes simplex virus inoculation in murine rete testis results in irreversible testicular damage
| Autor: | Andrey Yu Kulibin, Elena A Gushchina, Zavalishina Le, E. A. Malolina, Alla A. Kushch, V.A. Naumenko |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Human cytomegalovirus animal diseases viruses Biology medicine.disease_cause Virus Herpesviridae Pathology and Forensic Medicine Male infertility Mice Rete testis medicine Animals Simplexvirus Spermatogenesis Molecular Biology Infertility Male Rete Testis Sertoli Cells Herpes Simplex Original Articles Cell Biology medicine.disease Sertoli cell Spermatozoa Virology medicine.anatomical_structure Herpes simplex virus |
| Zdroj: | International Journal of Experimental Pathology. 95:120-130 |
| ISSN: | 0959-9673 |
| DOI: | 10.1111/iep.12071 |
| Popis: | According to the European Association of Urology (EAU), infertility affects 15% of reproductive-aged couples with roughly half of these cases being attributed to a male factor (EAU 2010). Accumulating evidence indicates that viral infections contribute to male infertility (Garolla et al. 2013). For example, mumps virus and human immunodeficiency virus (HIV) in patients with AIDS cause inflammatory reactions in the testis and subsequent impairment of spermatogenesis (Dejucq & Jegou 2001). Another widespread virus, herpes simplex virus (HSV-1, 2), has been found in the semen and testes of men, but data on its prevalence can vary across studies (Wald et al. 1999; Kapranos et al. 2003; Bezold et al. 2007). The presence of HSV-1, 2 in semen has been associated with a decrease in sperm concentration and actively motile spermatozoa, as well as an increased number of degenerating and immature germ cells, and abnormal spermatozoa (Kapranos et al. 2003; Bezold et al. 2007; Bocharova et al. 2007; Klimova et al. 2010). Moreover, a significant association has been found between detection of HSV in semen and infertility (Bocharova et al. 2007; Klimova et al. 2010). HSV and another member of the Herpesviridae, human cytomegalovirus (CMV), were found to replicate and cause damage to somatic and germ cells in testis organotypic culture (Naumenko et al. 2011a,b2011b). Despite the significance of these observations, it remains unclear whether HSV can cause testicular infection in vivo. A natural route of HSV entry into the testis is also yet to be established. It has been shown that HSV does not infect testes via the hematogenous route (Burgos et al. 2005). Additionally, in the case of interstitial injection, murine CMV has been detected in endothelial and Leydig cells, but not inside the seminiferous tubules. It is possible that Sertoli cells, by the production of anti-viral factors or by their junction system, prevent herpes virus occurrence in the seminiferous tubules (Tebourbi et al. 2001). The lack of a suitable animal model has hampered the investigations in this field. Here, we report the development of a mouse model of testicular HSV infection. In this model, the virus is injected into seminiferous tubules of adult mice via rete testis, mimicking the retrograde path of the virus from male urogenital tract into the testis. The aim was to characterize testicular HSV infection and clarify the influence of HSV on testis morphology and spermatogenesis. |
| Databáze: | OpenAIRE |
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