Molecular Identification and Targeted Quantitative Analysis of Medicinal Materials from Uncaria Species by DNA Barcoding and LC-MS/MS
| Autor: | Jing Qiao, Xiaojun Ma, Zuliang Luo, Jine Fu, Li-Xia Zhang, Shugen Wei, Zhong-Lian Zhang, Shengrong Cui |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Evaluation system
DNA Plant Pharmaceutical Science 01 natural sciences DNA barcoding Article Analytical Chemistry Indole Alkaloids Discriminatory power lcsh:QD241-441 Alkaloids lcsh:Organic chemistry Tandem Mass Spectrometry Drug Discovery Lc ms ms DNA Barcoding Taxonomic Physical and Theoretical Chemistry Medicine Chinese Traditional Molecular identification Uncaria species biology Traditional medicine quantitative analysis 010405 organic chemistry Organic Chemistry biology.organism_classification 0104 chemical sciences 010404 medicinal & biomolecular chemistry Uncaria Rhynchophylline Chemistry (miscellaneous) LC-MS/MS Molecular Medicine identification Quantitative analysis (chemistry) Chromatography Liquid |
| Zdroj: | Molecules, Vol 24, Iss 1, p 175 (2019) Molecules Volume 24 Issue 1 |
| ISSN: | 1420-3049 |
| Popis: | The genus Uncaria is an important source of traditional Chinese medicines with multiple therapeutic effects. The identification of the correct species and accurate determination of the contents of bioactive constituents are important for quality control of Uncaria medicinal materials. Here, an integrated evaluation system based on DNA barcoding for species identification and quantitative analysis by LC-MS/MS has been established. DNA barcoding based on the ITS2 barcode region showed sufficient discriminatory power to precisely identify 24 samples from seven Uncaria species. The length of the 24 ITS2 sequences of Uncaria samples is 227 bp, and 17 variation sites were detected. Additionally, the results of qualitative and quantitative chemical analyses by LC-MS/MS indicated that the chemical compositions of all Uncaria samples were similar while their contents of targeted alkaloids in samples from different species and origin areas were different. The contents of rhynchophylline (RC) and isorhynchophylline (IRC) were 2.9&ndash 1612 mg/kg and 2.60&ndash 1299 mg/kg in all tested samples, respectively. This study concludes that DNA barcoding should be used as the first screening step for Uncaria medicinal materials. Then, integration of DNA barcoding with chemical analyses should be applied in quality control of Uncaria medicinal materials in the medicinal industry. |
| Databáze: | OpenAIRE |
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