Characterization of tick organic anion transporting polypeptides (OATPs) upon bacterial and viral infections

Autor: Wenshuo Zhou, Vikas Taank, John F. Anderson, Xuran Zhuang, Utpal Pal, Hameeda Sultana, Girish Neelakanta
Rok vydání: 2018
Předmět:
Nymph
Organic anion transporting polypeptide
0301 basic medicine
Xanthurenates
Langat virus
030231 tropical medicine
Gene Expression
Organic Anion Transporters
Biology
Tick
Real-Time Polymerase Chain Reaction
Virus
Cell Line
Encephalitis Viruses
Tick-Borne
lcsh:Infectious and parasitic diseases
Microbiology
03 medical and health sciences
0302 clinical medicine
Xanthurenic acid
parasitic diseases
Gene expression
Animals
lcsh:RC109-216
Borrelia burgdorferi
Pathogen
Transaminases
Ixodes
Kynurenine aminotransferase
Research
Computational Biology
Sulfinpyrazone
bacterial infections and mycoses
biology.organism_classification
3. Good health
Organic anion-transporting polypeptide
030104 developmental biology
Infectious Diseases
Virus Diseases
Anti-vector vaccine
Ixodes scapularis
biology.protein
Arachnid Vectors
Parasitology
Anaplasma phagocytophilum
Zdroj: Parasites & Vectors, Vol 11, Iss 1, Pp 1-12 (2018)
Parasites & Vectors
ISSN: 1756-3305
DOI: 10.1186/s13071-018-3160-6
Popis: Background Ixodes scapularis organic anion transporting polypeptides (OATPs) play important roles in tick-rickettsial pathogen interactions. In this report, we characterized the role of these conserved molecules in ticks infected with either Lyme disease agent Borrelia burgdorferi or tick-borne Langat virus (LGTV), a pathogen closely related to tick-borne encephalitis virus (TBEV). Results Quantitative real-time polymerase chain reaction analysis revealed no significant changes in oatps gene expression upon infection with B. burgdorferi in unfed ticks. Synchronous infection of unfed nymphal ticks with LGTV in vitro revealed no significant changes in oatps gene expression. However, expression of specific oatps was significantly downregulated upon LGTV infection of tick cells in vitro. Treatment of tick cells with OATP inhibitor significantly reduced LGTV loads, kynurenine amino transferase (kat), a gene involved in the production of tryptophan metabolite xanthurenic acid (XA), levels and expression of several oatps in tick cells. Furthermore, bioinformatics characterization of OATPs from some of the medically important vectors including ticks, mosquitoes and lice revealed the presence of several glycosylation, phosphorylation and myristoylation sites. Conclusions This study provides additional evidence on the role of arthropod OATPs in vector-intracellular pathogen interactions. Electronic supplementary material The online version of this article (10.1186/s13071-018-3160-6) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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