Müllerian Inhibiting Substance in the Caudate Amphibian Pleurodeles waltl
| Autor: | Stéphane Flament, Nathalie di Clemente, Amand Chesnel, Hélène Dumond, Dominique Chardard, Jean-Yves Picard, Imane Al-Asaad |
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| Přispěvatelé: | Centre de Recherche en Automatique de Nancy (CRAN), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Université de Lorraine (UL)-Institut National de la Recherche Agronomique (INRA), Endocrinologie et Génétique de la Reproduction et du Développement, Université Paris-Sud - Paris 11 (UP11)-IFR13-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL) |
| Rok vydání: | 2013 |
| Předmět: |
Anti-Mullerian Hormone
Male Pleurodeles medicine.medical_specialty Sex Differentiation endocrine system diseases Mullerian Ducts MESH: Amphibian Proteins - Animals - Anti-Mullerian Hormone - Female - Gene Expression Regulation - Developmental - In Situ Hybridization - Larva - Liver - Male - Metamorphosis - Biological - Mullerian Ducts - Organ Culture Techniques - Ovary - Phylogeny - Pleurodeles - Protein Structure - Tertiary - RNA - Messenger - Sertoli Cells - Sex Differentiation - Testis 030209 endocrinology & metabolism [SDV.BC]Life Sciences [q-bio]/Cellular Biology Mullerian duct regression Amphibian Proteins [SPI.AUTO]Engineering Sciences [physics]/Automatic 03 medical and health sciences Organ Culture Techniques 0302 clinical medicine Endocrinology Germ cell proliferation Internal medicine Testis otorhinolaryngologic diseases medicine Animals RNA Messenger In Situ Hybridization Phylogeny 030304 developmental biology 0303 health sciences Sertoli Cells Sexual differentiation biology Ovary Metamorphosis Biological Gene Expression Regulation Developmental Anti-Müllerian hormone Sex reversal biology.organism_classification Protein Structure Tertiary Liver Larva biology.protein Female Male sex differentiation |
| Zdroj: | Endocrinology Endocrinology, Endocrine Society, 2013, 154 (10), pp.3931-3936. ⟨10.1210/en.2013-1229⟩ |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/en.2013-1229 |
| Popis: | International audience; Müllerian inhibiting substance (MIS, also known as anti-Müllerian hormone), is a key factor of male sex differentiation in vertebrates. In amniotes, it is responsible for Müllerian duct regression in male embryos. In fish, despite the absence of Müllerian ducts, MIS is produced and controls germ cell proliferation during gonad differentiation. Here we show for the first time the presence of MIS in an amphibian species, Pleurodeles waltl. This is very astonishing because in caudate amphibians, Müllerian ducts do not regress in males. Phylogenetic analysis of MIS P. waltl ortholog revealed that the deduced protein segregates with MIS from other vertebrates and is clearly separated from other TGF-β family members. In larvae, MIS mRNA was expressed at higher levels in the developing testes than in the ovaries. In the testis, MIS mRNA expression was located within the lobules that contain Sertoli cells. Besides, expression of MIS was modified in the case of sex reversal: it increased after masculinizing heat treatment and decreased after estradiol feminizing exposure. In addition to the data obtained recently in the fish medaka, our results suggest that the role of MIS on Müllerian ducts occurred secondarily during the course of evolution. |
| Databáze: | OpenAIRE |
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