Effects of micro-encapsulation on morphology and endocrine function of cryopreserved neonatal porcine islet-like cell clusters
| Autor: | Gizou Nakagawara, Makoto Murakami, Akio Yamaguchi, Taizou Kobayashi, Hirohide Satou, Toshihisa Kimura, Hiroo Iwata |
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| Rok vydání: | 2000 |
| Předmět: |
Male
medicine.medical_specialty Cell Survival Swine medicine.medical_treatment Xenotransplantation Drug Compounding Islets of Langerhans Transplantation Biology Cryopreservation Islets of Langerhans In vivo Internal medicine Insulin Secretion medicine Animals Insulin Incubation Cells Cultured Transplantation Islet cell transplantation geography geography.geographical_feature_category Islet Survival Rate Endocrinology Animals Newborn Collagenase Female medicine.drug |
| Zdroj: | Transplantation. 70(8) |
| ISSN: | 0041-1337 |
| Popis: | BACKGROUND For the success of clinical islets transplantation, the development of a long-term storage method is necessary. However, the structure of digested islets is scanty for culture and cryopreservation. In this study, the effect of micro-encapsulation to cryopreserved porcine islet-like cell clusters (ICCs) was investigated. METHODS The ICCs prepared from neonatal pigs by collagenase digestion and culture technique were cryopreserved and micro-encapsulated in 5% agarose membranes. After cryopreservation, ICC cultured without encapsulation (group A) and cultured with encapsulation (group B) were assessed by comparison with no cryopreserved ICC (control) both in vitro by static incubation test and in vivo in a xenotransplantation study. RESULTS Micro-encapsulation was able to maintain the fine morphology and the number of ICCs of group B after 7 days of culture. There were not significant differences in insulin secretion of group B and control on day 1 and 7 of culture (1 day:11+/-0.99, 7 days: 5.30+/-1.08 microU/ICC/hr NS versus control). On day 7 of culture, the retrieval rate of group B (105.2+/-9.8%) is obviously higher compared with group A (63.0+/-6.3%). In the xenotransplatation model, the ICCs of group B showed long survival time (7.9+/-0.4 weeks) and good transplantation effect. CONCLUSION Our study suggests that micro-encapsulation is one of the useful method for cryopreserved ICC to maintain the fine morphology and effectively recover the endocrine function. |
| Databáze: | OpenAIRE |
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