Secretome of Senescent Adipose-Derived Mesenchymal Stem Cells Negatively Regulates Angiogenesis

Autor: Andrey Ratushnyy, Ludmila Buravkova, M. I. Ezdakova
Rok vydání: 2020
Předmět:
Adult
animal structures
Angiogenesis
Adipose tissue
Neovascularization
Physiologic
Catalysis
Chorioallantoic Membrane
Article
lcsh:Chemistry
Inorganic Chemistry
Neovascularization
Paracrine signalling
replicative senescence
angiogenesis
Cell Movement
Paracrine Communication
medicine
Human Umbilical Vein Endothelial Cells
Humans
Physical and Theoretical Chemistry
Angiogenic Proteins
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
Tissue homeostasis
Cells
Cultured
Cellular Senescence
Cell Proliferation
Matrigel
Chemistry
Organic Chemistry
Mesenchymal stem cell
senescence-associated secretory phenotype (SASP)
Mesenchymal Stem Cells
General Medicine
Middle Aged
Computer Science Applications
Cell biology
adipose-derived mesenchymal stem cells (ASCs)
Chorioallantoic membrane
lcsh:Biology (General)
lcsh:QD1-999
Female
medicine.symptom
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 5
International Journal of Molecular Sciences, Vol 21, Iss 5, p 1802 (2020)
ISSN: 1422-0067
Popis: Nowadays, paracrine regulation is considered as a major tool of mesenchymal stem cell (MSC) involvement in tissue repair and renewal in adults. Aging results in alteration of tissue homeostasis including neovascularization. In this study, we examined the influence of replicative senescence on the angiogenic potential of adipose-derived MSCs (ASCs). Angiogenic activity of conditioned medium (CM) from senescent and &ldquo
young&rdquo
ASCs was evaluated in chorioallantoic membrane (CAM) assay in ovo using Japanese quail embryos. Also, the formation of capillary-like tubes by human umbilical vein endothelial cells (HUVECs) in 3D basement membrane matrix &lsquo
&lsquo
Matrigel&rsquo
&rsquo
and HUVEC migration capacity were analyzed. Multiplex, dot-blot and gene expression analysis were performed to characterize transcription and production of about 100 angiogenesis-associated proteins. The results point to decreased angiogenic potential of senescent ASC secretome in ovo. A number of angiogenesis-associated proteins demonstrated elevation in CM after long-term cultivation. Meanwhile, VEGF (key positive regulator of angiogenesis) did not change transcription level and concentration in CM. Increasing both pro- (FGF-2, uPA, IL-6, IL-8 etc.) and antiangiogenic (IL-4, IP-10, PF4, Activin A, DPPIV etc.) factors was observed. Some proangiogenic genes were downregulated (IGF1, MMP1, TGFB3, PDGFRB, PGF). Senescence-associated secretory phenotype (SASP) modifications after long-term cultivation lead to attenuation of angiogenic potential of ASC.
Databáze: OpenAIRE
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