Oxidative Stress Implication After Prolonged Storage Donor Heart with Blood Versus Crystalloid Cardioplegia and Reperfusion Versus Static Storage
| Autor: | Jean-Paul Albertini, Sylvie Dever, Jacques Delattre, Noelle Vignat, Jean-Yves Artigou, Patrick Nataf, Jean-Christophe Charniot, Alain Pavie, Jean-Jacques Monsuez, Dominique Bonnefont-Rousselot |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Potassium Compounds
medicine.medical_treatment Heart preservation Hemodynamics medicine.disease_cause Thiobarbituric Acid Reactive Substances Blood Transfusion Autologous Ventricular Pressure medicine TBARS Animals Vitamin E Lactic Acid Vitamin A Cryopreservation Heart transplantation Glutathione Peroxidase Sheep Superoxide Dismutase business.industry Myocardium Graft Survival Arrhythmias Cardiac Organ Preservation Blood flow beta Carotene medicine.disease Myocardial Contraction Transplantation Oxidative Stress Reperfusion Injury Anesthesia Heart Arrest Induced Heart Transplantation Surgery Lipid Peroxidation business Reperfusion injury Oxidative stress |
| Zdroj: | Journal of Surgical Research. 160:308-314 |
| ISSN: | 0022-4804 |
| Popis: | Several factors are known to limit cardiac transplantation, such as number of donors, quality of cardiac graft preservation, and ischemia-reperfusion injury. Some mechanisms of reperfusion injury are now recognized; they include oxygen free radical (OFR), white blood cells activation, changes in calcium influx, alteration of microvascular blood flow, and sympathetic activation. The goal of this study was to assess the effects of two types of cardioplegia with long-term storage, either static or continuous perfusion, in 30 isolated sheep hearts as a model for heart transplantation. We examined myocardial function, histology, ischemic damage, and markers of oxidative stress. Two types of cardioplegia and storage conditions using a Langendorff reperfusion were studied in a combined approach: crystalloid (CP) [groups I and III] or cold oxygenated autologous blood (BC) [groups II and IV], immediate storage during 8h in profound hypothermia (groups I and II), or reperfused with crystalloid (group III), or blood cardioplegia (group IV). All perfusate samples were drawn from the coronary sinus. Lactate levels increased progressively in groups I, II, and IV, but not in group III, as no significant elevation was shown [90 min: 13.6 ± 1.7 versus 5.2 ± 1.0 mmol/L (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect