Plasma Membrane Cyclic Nucleotide Gated Calcium Channels Control Land Plant Thermal Sensing and Acquired Thermotolerance
| Autor: | Pierre Goloubinoff, Younousse Saidi, Frans J. M. Maathuis, America Farinia Henriquez Cuendet, Andrija Finka |
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| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
Adaptation Biological Arabidopsis Cyclic Nucleotide-Gated Cation Channels Plant Science Physcomitrella patens 01 natural sciences 03 medical and health sciences Cyclic nucleotide chemistry.chemical_compound Heat shock protein Botany Arabidopsis thaliana Patch clamp Heat shock Research Articles 030304 developmental biology 0303 health sciences biology Voltage-dependent calcium channel Arabidopsis Proteins Calcium channel Cell Membrane Cell Biology biology.organism_classification Bryopsida chemistry Biophysics 010606 plant biology & botany |
| Zdroj: | The Plant cell |
| ISSN: | 1532-298X 1040-4651 |
| DOI: | 10.1105/tpc.112.095844 |
| Popis: | Typically at dawn on a hot summer day land plants need precise molecular thermometers to sense harmless increments in the ambient temperature to induce a timely heat shock response (HSR) and accumulate protective heat shock proteins in anticipation of harmful temperatures at mid day. Here we found that the cyclic nucleotide gated calcium channel (CNGC) CNGCb gene from Physcomitrella patens and its Arabidopsis thaliana ortholog CNGC2 encode a component of cyclic nucleotide gated Ca(2+) channels that act as the primary thermosensors of land plant cells. Disruption of CNGCb or CNGC2 produced a hyper thermosensitive phenotype giving rise to an HSR and acquired thermotolerance at significantly milder heat priming treatments than in wild type plants. In an aequorin expressing moss CNGCb loss of function caused a hyper thermoresponsive Ca(2+) influx and altered Ca(2+) signaling. Patch clamp recordings on moss protoplasts showed the presence of three distinct thermoresponsive Ca(2+) channels in wild type cells. Deletion of CNGCb led to a total absence of one and increased the open probability of the remaining two thermoresponsive Ca(2+) channels. Thus CNGC2 and CNGCb are expected to form heteromeric Ca(2+) channels with other related CNGCs. These channels in the plasma membrane respond to increments in the ambient temperature by triggering an optimal HSR leading to the onset of plant acquired thermotolerance. |
| Databáze: | OpenAIRE |
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