Left ventricular function measurements in a mouse myocardial infarction model Comparison between 3D-echocardiography and ECG-gated SPECT

Autor: Felix M. Mottaghy, Matthias Bauwens, Roel Wierts, Geert Hendrikx, Mark J. Post
Přispěvatelé: Promovendi CD, Beeldvorming, RS: NUTRIM - R1 - Metabolic Syndrome, Promovendi ODB, MUMC+: DA BV Nucleaire Geneeskunde (9), RS: GROW - R3 - Innovative Cancer Diagnostics & Therapy, RS: CARIM - R3.08 - Regenerative and reconstructive medicine for vascular disease, Fysiologie
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Male
Technetium Tc 99m Sestamibi
medicine.medical_specialty
Gated SPECT
Echocardiography
Three-Dimensional
030204 cardiovascular system & hematology
Anterior Descending Coronary Artery
Sensitivity and Specificity
030218 nuclear medicine & medical imaging
03 medical and health sciences
Mice
Ventricular Dysfunction
Left
left ventricular function
0302 clinical medicine
Internal medicine
medicine
3D-echocardiography
Animals
Humans
Radiology
Nuclear Medicine and imaging
cardiovascular diseases
Myocardial infarction
Ejection fraction
business.industry
ECG-gated SPECT
Reproducibility of Results
Stroke Volume
General Medicine
Stroke volume
medicine.disease
Disease Models
Animal
myocardial infarction
Cardiology
Radiopharmaceuticals
Ligation
business
Cardiac-Gated Single-Photon Emission Computer-Assisted Tomography
Perfusion
Zdroj: Nuklearmedizin, 55(3), 115-122. Georg Thieme Verlag
ISSN: 0029-5566
Popis: SummaryAim: To assess the accuracy of ECG-gated micro (µ)-SPECT in a mouse myocardial infarction (MI) model in comparison to 3D-echocardiography. Animals, methods: In a mouse (Swiss mice) MI model we compared the accuracy of technetium-99m sestamibi (99mTc-sestamibi) myocardial perfusion, electrocardiogram (ECG) gated µSPECT to 3D-echocardiography in determining left ventricular function. 3D-echocardiography and myocardial perfusion ECG-gated µSPECT data were acquired in the same animal at baseline (n = 11) and 7 (n = 8) and 35 (n = 9) days post ligation of the left anterior descending coronary artery (LAD). Sham operated mice were used as a control (8, 6 and 7 mice respectively). Additionally, after day 35 µSPECT scans, hearts were harvested and 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) staining and autoradiography was performed to determine infarct size. Results: In both infarcted and sham-operated mice we consistently found comparable values for the end-diastolic volume (EDV), end-systolic volume (ESV) and ejection fraction (EF) obtained by 3D-echocardiography and ECG-gated µSPECT. Excellent correlations between measurements from 3D-echocardiography and ECG-gated µSPECT were found for EDV, ESV and EF (r = 0.9532, r = 0.9693 respectively and r = 0.9581) in infarcted mice. Furthermore, comparable infarct size values were found at day 35 post MI by TTC staining and autoradiography (27.71 ± 1.80% and 29.20 ± 1.18% with p = 0.43). Conclusion: We have demonstrated that ECG-gated µSPECT imaging provides reliable left ventricular function measurements in a mouse MI model. Obtained results were comparable to the highly accurate 3D-echocardiography. This, in addition to the opportunity to simultaneously image multiple biological processes during a single acquisition makes µSPECT imaging a serious option for studying cardiovascular disease in small animals.
Databáze: OpenAIRE
Externí odkaz: