Increased oxidative stress and anaerobic energy release, but blunted Thr172-AMPKα phosphorylation, in response to sprint exercise in severe acute hypoxia in humans
| Autor: | David Morales-Alamo, Lorena Rodríguez-García, Borja Guerra, Alfredo Santana, Mario Guerrero, Amelia Guadalupe-Grau, Jose A. L. Calbet, Maria Roser Cusso, Jesús Gustavo Ponce-González, Cecilia Dorado |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Blood Glucose Male Threonine medicine.medical_specialty Time Factors Anaerobic Threshold Physiology Biopsy AMP-Activated Protein Kinases Protein Serine-Threonine Kinases medicine.disease_cause Severity of Illness Index Quadriceps Muscle Running Protein Carbonylation Young Adult Oxygen Consumption Mediator AMP-Activated Protein Kinase Kinases Sirtuin 1 AMP-activated protein kinase Physiology (medical) Internal medicine medicine Humans Insulin Lactic Acid Muscle Strength Phosphorylation Hypoxia Protein kinase A Exercise biology GTPase-Activating Proteins AMPK Blood Proteins Metabolism NAD Oxidative Stress Endocrinology Acute Disease Exercise Test biology.protein Energy Metabolism Anaerobic exercise Oxidative stress Muscle Contraction |
| Zdroj: | Journal of Applied Physiology. 113:917-928 |
| ISSN: | 1522-1601 8750-7587 |
| DOI: | 10.1152/japplphysiol.00415.2012 |
| Popis: | AMP-activated protein kinase (AMPK) is a major mediator of the exercise response and a molecular target to improve insulin sensitivity. To determine if the anaerobic component of the exercise response, which is exaggerated when sprint is performed in severe acute hypoxia, influences sprint exercise-elicited Thr172-AMPKα phosphorylation, 10 volunteers performed a single 30-s sprint (Wingate test) in normoxia and in severe acute hypoxia (inspired Po2: 75 mmHg). Vastus lateralis muscle biopsies were obtained before and immediately after 30 and 120 min postsprint. Mean power output and O2 consumption were 6% and 37%, respectively, lower in hypoxia than in normoxia. O2 deficit and muscle lactate accumulation were greater in hypoxia than in normoxia. Carbonylated skeletal muscle and plasma proteins were increased after the sprint in hypoxia. Thr172-AMPKα phosphorylation was increased by 3.1-fold 30 min after the sprint in normoxia. This effect was prevented by hypoxia. The NAD+-to-NADH.H+ ratio was reduced (by 24-fold) after the sprints, with a greater reduction in hypoxia than in normoxia ( P < 0.05), concomitant with 53% lower sirtuin 1 (SIRT1) protein levels after the sprint in hypoxia ( P < 0.05). This could have led to lower liver kinase B1 (LKB1) activation by SIRT1 and, hence, blunted Thr172-AMPKα phosphorylation. Ser485-AMPKα1/Ser491-AMPKα2 phosphorylation, a known negative regulating mechanism of Thr172-AMPKα phosphorylation, was increased by 60% immediately after the sprint in hypoxia, coincident with increased Thr308-Akt phosphorylation. Collectively, our results indicate that the signaling response to sprint exercise in human skeletal muscle is altered in severe acute hypoxia, which abrogated Thr172-AMPKα phosphorylation, likely due to lower LKB1 activation by SIRT1. |
| Databáze: | OpenAIRE |
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