Influence of technetium-99m-labeling conditions on physico-chemical and related biological properties of an acylated poly-galactosidic macrophage targeting agent for inflammation imaging
| Autor: | F. Perin, Oumar N'Doye, Mamadou Mbodj, Alain Le Pape, Laurence Routledge, Gerard Normier |
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| Rok vydání: | 1996 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Biodistribution Magnetic Resonance Spectroscopy Chemical Phenomena chemistry.chemical_element Technetium Scintigraphy Colloid Glycolipid Drug Stability In vivo medicine Animals Radiology Nuclear Medicine and imaging Tissue Distribution Rats Wistar Radionuclide Imaging Inflammation medicine.diagnostic_test Chemistry Physical Arthritis Macrophages Phosphorus Organotechnetium Compounds In vitro Rats chemistry Isotope Labeling Biophysics Molecular Medicine Rabbits Glycolipids Radiopharmaceuticals Technetium-99m |
| Zdroj: | Nuclear medicine and biology. 23(8) |
| ISSN: | 0969-8051 |
| Popis: | The potential of 99m-Tc-J001 for the investigation of inflammatory lesions via the targeting of recruited macrophages (M phi) has already been documented in several experimental models and in human diseases. To achieve a functional imaging of inflammation via M phi targeting, minimal labeled colloid content and high in vivo stability of 99mTc-J001 are essential. The actual specificity of such scintigraphy is closely dependent upon the radiolabeling of only the J001 molecules available for M phi targeting. To develop an appropriate radiopharmaceutical kit, optimization of the labeling conditions was achieved from a series of pilot formulations that were evaluated for radiolabeling efficiency and both in vitro and in vivo 99mTc-J001 stability. Colloids were characterized using autocorrelation spectroscopy and multiangle laser-light scattering, radioactive colloid content of the formulations being deduced from biodistribution studies. This work has made possible the definition of a formulation exhibiting a radiolabeling yield > 97.0%, associated with in vivo stability and minimal colloid formation, thus greatly enhancing the specificity of such macrophage scintigraphy. |
| Databáze: | OpenAIRE |
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