Cytotoxic and apoptotic effects of endemic Centaurea fenzlii Reichardt on the MCF-7 breast cancer cell line
| Autor: | Ümit Yirtici, Aysun Ergene, Mehmet Sarimahmut, Fatih Göger |
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| Přispěvatelé: | Uludağ Üniversitesi/Fen-Edebiyat Fakültesi/Biyoloji Bölümü., Sarımahmut, Mehmet, AAG-8288-2021, Kırıkkale Üniversitesi, Anadolu Üniversitesi, Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, Göğer, Fatih |
| Rok vydání: | 2017 |
| Předmět: |
Centaurea
Antioxidant Sesquiterpene Lactone 0301 basic medicine Endemic Centaurea fenzlii Reichardt Dichloromethane extract- ethyl acetate fraction Physiology Centaurea fenzlii Proliferation Apoptotic effect Asteraceae Biology Pharmacology Microbiology Extracts 03 medical and health sciences 0302 clinical medicine Breast cancer cell line Genetics Cytotoxic T cell Growth-inhibition Molecular Biology Life sciences & biomedicine - other topics MTT assay Toxicity Endemic Centaurea fenzlii Reichardt MCF-7 dichloromethane extract- ethyl acetate fraction cytotoxicity apoptotic effect 030206 dentistry Cell Biology Cytotoxicityap 030104 developmental biology MCF-7 Apoptosis Constituents cytotoxicity General Agricultural and Biological Sciences ATP assay |
| Zdroj: | Volume: 41, Issue: 2 370-377 Turkish Journal of Biology |
| ISSN: | 1303-6092 1300-0152 |
| Popis: | WOS: 000400179800013 The main purpose of this study was to analyze the cytotoxic activity of an extract obtained from Centaurea fenzlii Reichardt, and the fractions eluted from this extract, in breast cancer cells. After isolation and structural analysis of the fractions were conducted, a meaningful cytotoxic effect was indicated. The goal of the analysis was to reveal the mechanism by which this effect occurs through researching the apoptotic side of these fractions and determining the amount of several proteins that are the products of the genes. Test substances were applied to breast cancer cells and the inhibitory concentration value 50 (IC50) that caused a cytotoxic effect was determined using MTT and ATP assays. The Centaurea fenzlii Reichardt dichloromethane extracts-ethyl acetate fractions (CFDCM-EAF) exhibited a stronger growth-inhibitory effect on MCF-7 cells (45.771 mu g/mL). The apoptotic effect was studied using double staining and flow cytometry. The death rate in the cells treated with the CFDCM-EAF IC50 dose was approximately 90%: 9.2% living cells, 22.8% necrotic cells, 62.3% late apoptotic cells, and 5.8% early apoptotic cells. Structural analysis of the CFDCM-EAF, which indicated significant cytotoxic effects, was performed using chromatographic methods. Hispidulin was the major component of the CFDCM-EAF by LC-APCI-MS/MS analysis. Scientific and Technological Research Council of Turkey (TUBITAK) [110T905] This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK Project No. 110T905). The authors would like to thank E Ulukaya (Prof in Faculty of Medicine at Uludag University) and N Kirimer (Prof in Faculty of Pharmacy at Anadolu University) for their comments on experiments, and F Yilmaz for her technical assistance. |
| Databáze: | OpenAIRE |
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