Gene copy number analysis in malignant pleural mesothelioma using oligonucleotide array CGH
| Autor: | Pamela Lindholm, Hanna Vauhkonen, A G Nicholson, Sisko Anttila, Vuokko L. Kinnula, Sakari Knuutila, Kaisa Salmenkivi |
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| Rok vydání: | 2007 |
| Předmět: |
Mesothelioma
Genome Human Pleural Neoplasms Gene Dosage Nucleic Acid Hybridization Locus (genetics) Biology medicine.disease_cause Molecular biology Genome Nucleic acid thermodynamics CDKN2A CDKN2B Genetics medicine Humans Copy-number variation Carcinogenesis Molecular Biology Genetics (clinical) Oligonucleotide Array Sequence Analysis Comparative genomic hybridization |
| Zdroj: | Cytogenetic and Genome Research. 119:46-52 |
| ISSN: | 1424-859X 1424-8581 |
| DOI: | 10.1159/000109618 |
| Popis: | Conventional cytogenetic analyses and comparative genomic hybridization have revealed a complex and even chaotic nature of chromosomal aberrations in pleural malignant mesothelioma (MM). We set out to describe the complex gene copy number changes and screen for novel genetic aberrations using a high-density oligonucleotide microarray platform for comparative genomic hybridization (aCGH) of a series of 26 well-characterized MM tumor samples. The number of copy number changes varied from zero to 40 per sample. Gene copy number losses predominated over gains, and the most frequent region of loss was 9p21.3 (17/26 cases), the locus of CDKN2A and CDKN2B, both known to be commonly lost in MM. The most recurrent minimal regions of losses were 1p31.1→ p13.2, 3p22.1→p14.2, 6q22.1, 9p21.3, 13cen→q14.12, 14q22.1→qter, and 22qcen→q12.3. Previously unreported gains included 9p13.3, 7p22.3→p22.2, 12q13.3, and 17q21.32→qter. The results suggest that gene copy number losses are a major mechanism of MM carcinogenesis and reveal a recurrent pattern of copy number changes in MM. |
| Databáze: | OpenAIRE |
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