ATRX-mediated chromatin association of histone variant macroH2A1 regulates α-globin expression
| Autor: | Ratnakumar, K, Duarte, LF, LeRoy, G, Hasson, D, Smeets, D, Vardabasso, C, Bönisch, C, Zeng, T, Xiang, B, Zhang, DY, Li, H, Wang, X, Hake, SB, Schermelleh, L, Garcia, BA, Bernstein, E |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
X-linked Nuclear Protein
Chromatin remodeling Histones Research Communication Erythroid Cells alpha-Globins alpha-Thalassemia Genetics Humans Histone code ChIA-PET ATRX biology DNA Helicases Nuclear Proteins Telomere Subtelomere Molecular biology Phenotype Chromatin HEK293 Cells Histone Gene Expression Regulation Gene Knockdown Techniques Mental Retardation X-Linked biology.protein K562 Cells HeLa Cells Developmental Biology |
| DOI: | 10.1101/gad.179416.111 |
| Popis: | The histone variant macroH2A generally associates with transcriptionally inert chromatin; however, the factors that regulate its chromatin incorporation remain elusive. Here, we identify the SWI/SNF helicase ATRX (α-thalassemia/ MR, X-linked) as a novel macroH2A-interacting protein. Unlike its role in assisting H3.3 chromatin deposition, ATRX acts as a negative regulator of macroH2A's chromatin association. In human erythroleukemic cells deficient for ATRX, macroH2A accumulates at the HBA gene cluster on the subtelomere of chromosome 16, coinciding with the loss of α-globin expression. Collectively, our results implicate deregulation of macroH2A's distribution as a contributing factor to the α-thalassemia phenotype of ATRX syndrome. © 2012 by Cold Spring Harbor Laboratory Press. |
| Databáze: | OpenAIRE |
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