Localization of Sarcoma Xenografts in Nude Mice with Indium-111-Labeled Monoclonal Antibodies
| Autor: | A.Dwight Lopes, Michael J. Blend, Robert W. Atcher, Tzu-Chieh Chao, Tapas K. Das Gupta, John A. Greager |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
Pathology medicine.medical_specialty Biodistribution medicine.drug_class Blood pool Transplantation Heterologous Mice Nude Negative control Kidney Monoclonal antibody Mice Antigens Neoplasm Tumor Cells Cultured medicine Animals Humans Tissue Distribution Binding site biology business.industry Indium Radioisotopes Antibodies Monoclonal Pentetic Acid Carbohydrate medicine.disease Molecular biology Liver biology.protein Surgery Sarcoma Experimental Sarcoma Antibody business Oligopeptides Neoplasm Transplantation Spleen |
| Zdroj: | Journal of Surgical Research. 55:38-43 |
| ISSN: | 0022-4804 |
| Popis: | A labeling method utilizing modified carbohydrate moieties in antibody heavy chains as radionucleotide binding sites was evaluated. Murine anti-sarcoma monoclonal antibody (MAb 19-24) was labeled with Indium-111 (111In) using this technique and subcutaneous human sarcoma xenografts were successfully localized in nude mice. A nonspecific monoclonal antibody BL-3 was used as a negative control. Tumor-to-blood ratios of radioactivity in the mice injected with 111Inlabeled MAb 19-24 were significantly (P < 0.05) higher than those obtained with nonspecific MAb BL-3. Calculations of percentage injected dose of radioactivity per gram tissue showed relatively high specific uptake of MAb 19-24 in sarcoma xenografts. Radioactivity cleared from the blood rapidly and hepatic uptake of 111In-labeled antibodies was found to be relatively low. Biodistribution studies in normal mice with 111In-labeled antibodies showed only blood pool activity with no significant concentration of activity into organs. Therefore, immunoreactivity of the antibodies was retained after 111In-labeling utilizing this new technique, allowing specific binding of radiolabeled MAb to tumor xenografts with relatively low hepatic uptake. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|