NMR Spectroscopy-based Metabolomics of Drosophila Model of Huntington’s Disease Suggests Altered Cell Energetics
| Autor: | Ashwani Kumar Thakur, Neeraj Sinha, Raj Kumar Sharma, Pradip Sinha, Thamarailingam Athilingam, Virender Singh |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Magnetic Resonance Spectroscopy Transgene Metabolite Cell Energetics Biology Nicotinamide adenine dinucleotide Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Metabolomics Huntington's disease medicine Huntingtin Protein Animals Humans Neurons General Chemistry NAD medicine.disease Disease Models Animal Drosophila melanogaster Huntington Disease 030104 developmental biology chemistry NAD+ kinase Energy Metabolism Peptides 030217 neurology & neurosurgery |
| Zdroj: | Journal of Proteome Research. 16:3863-3872 |
| ISSN: | 1535-3907 1535-3893 |
| Popis: | Huntington’s disease (HD) is a neurodegenerative disorder induced by aggregation of the pathological form of Huntingtin protein that has expanded polyglutamine (polyQ) repeats. In the Drosophila model, for instance, expression of transgenes with polyQ repeats induce HD-like pathologies progressively correlating with the increasing lengths of these repeats. Previous studies on both animal models and clinical samples have revealed metabolite imbalances during HD progression. To further explore the physiological processes linked to metabolite imbalances during HD, we have investigated 1D 1H NMR spectroscopy-based metabolomics profile of Drosophila HD model. Using multivariate analysis (PCA and PLS-DA) of metabolites obtained from methanolic extracts of fly heads displaying retinal deformations due to polyQ overexpression, we show that the metabolite imbalance during HD are likely to affect cell energetics. Six out of the 35 metabolites analyzed, namely, nicotinamide adenine dinucleotide (NAD), lactate, pyruv... |
| Databáze: | OpenAIRE |
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