The effect of horse serum on in vitro development of porcine parthenogenetic embryos
| Autor: | Sung Woo Kim, Jin-Ki Park, Jong-Ju Park, In-Sul Hwang, Jae Gyu Yoo, Kyung-Woon Kim, Kuppannan Gobianand, Sung June Byun, Hee Kyoung Chung |
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| Rok vydání: | 2012 |
| Předmět: |
Serum
Swine Parthenogenesis Embryonic Development Apoptosis Biology Andrology Embryo Culture Techniques chemistry.chemical_compound Endocrinology medicine In Situ Nick-End Labeling Animals Blastocyst Horses Cytochalasin B Serum Albumin Embryogenesis Albumin Embryo Follicular fluid In vitro Follicular Fluid medicine.anatomical_structure chemistry embryonic structures Immunology Oocytes Animal Science and Zoology Cattle Female Fetal bovine serum Developmental Biology |
| Zdroj: | Reproductive biology. 12(1) |
| ISSN: | 2300-732X |
| Popis: | SUMMARY The objective of this study was to examine the effect of different sera and serum-like substances on the preimplantation development of porcine parthenogenetic embryos. Chemically activated (calcium ionophore A23187+cytochalasin B) pig oocytes were pre-cultured for five days. On day 5, the parthenogenetic embryos were treated with porcine follicular fluid (PFF), fetal bovine serum (FBS), horse serum (HS) or porcine serum albumin (PSA), and were cultured two more days. Horse serum was found to be the most effective protein source in enhancing parthenote development judging by blastocyst formation and hatching. Next, three different concentrations of HS (10, 20 and 30%) were used to determine the optimal HS concentration needed to improve the development of porcine parthenogenetic embryos. All HS concentrations increased the blastocyst cell number and decreased the incidence of blastocyst apoptotic cells with 20% being the most effective. In conclusion, horse serum enhanced parthenogenetic embryo development and the quality of porcine parthenogenetic embryos. |
| Databáze: | OpenAIRE |
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