Determinants of RNA quality from FFPE samples
| Autor: | Martin Schlumpberger, Andreas Missel, Silke von Ahlfen, Klaus Bendrat |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Analyte
Tissue Fixation lcsh:Medicine Computational biology Fixation time Biology Bioinformatics Specimen Handling Formaldehyde Animals Paraffin embedding Rats Wistar lcsh:Science Paraffin Embedding Multidisciplinary Reverse Transcriptase Polymerase Chain Reaction Microarray analysis techniques lcsh:R RNA Genetics and Genomics/Gene Expression Pathology/Molecular Pathology Rats Gene expression profiling lcsh:Q RNA extraction Research Article |
| Zdroj: | PLoS ONE, Vol 2, Iss 12, p e1261 (2007) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The large archives of formalin-fixed paraffin-embedded (FFPE) tissue specimens that exist are a highly valuable source of sample material for molecular biological analysis, including gene expression profiling. However, current data on adverse effects of standard pathological practice on the usefulness of biomolecular analytes obtained from such archived specimens is largely anecdotal. Here, we present a systematic examination of the most relevant parameters for integrity and useability of RNA obtained from FFPE samples, including storage time and conditions, fixation time, and specimen size. The results are particularly relevant for any application relying on cDNA synthesis as an initial step of the procedure, such as RT-PCR, and microarray analysis. |
| Databáze: | OpenAIRE |
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