Evaluation of a Rapid Whole Blood ELISA for Quantification of Troponin I in Patients with Acute Chest Pain
| Autor: | Christopher Heeschen, Britta U. Goldmann, Guido Matschuck, Lukas Langenbrink, Christian W. Hamm |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Thorax Chest Pain medicine.medical_specialty Percentile Point-of-Care Systems Fluoroimmunoassay Clinical Biochemistry Myocardial Infarction Enzyme-Linked Immunosorbent Assay Chest pain Internal medicine Troponin I medicine Humans Angina Unstable Myocardial infarction biology business.industry Unstable angina Biochemistry (medical) Middle Aged medicine.disease Troponin Surgery ROC Curve Acute Disease Ambulatory biology.protein Cardiology Female medicine.symptom business Biomarkers |
| Zdroj: | Clinical Chemistry. 45:1789-1796 |
| ISSN: | 1530-8561 0009-9147 |
| Popis: | Background: Troponin I (cTnI) provides important prognostic information in patients with chest pain. We wished to evaluate a rapid, whole-blood analyzer for quantitative point-of-care testing.Methods: A quantitative point-of-care test system (Stratus CS®; Dade-Behring) for cTnI with an incorporated centrifuge was evaluated in 412 patients with chest pain less than 12 h.Results: Results were available within 15 min. CVs were 4.5% at 0.1 μg/L, 4.2% at 0.25 μg/L, and 6.5% at 0.82 μg/L. The detection limit was 0.01 μg/L. The 97.5% percentile in a healthy population was 0.08 μg/L. Based on ROC curve analysis, a threshold of 0.15 μg/L was calculated for the detection of acute myocardial infarction (AMI). With it, sensitivity for the detection of patients with AMI (n = 62) was 63% at arrival and 98% after 4 h (Stratus II®, 48% and 85%, respectively; P Conclusion: The Stratus CS provided better analytical performance and comparable or better prognostic information than the Stratus II. |
| Databáze: | OpenAIRE |
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