A highly repeated retropseudogene-like sequence in DNA of the redbreasted merganser (Mergus serrator)
| Autor: | K. P. Mchugh, C. S. Madsen, S. R. De Kloet |
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| Rok vydání: | 1990 |
| Předmět: |
Molecular Sequence Data
EcoRI Biology Molecular cloning law.invention Birds chemistry.chemical_compound law Genetics Animals Mergus serrator Cloning Molecular Repetitive Sequences Nucleic Acid Genomic organization Base Sequence Nucleic acid sequence DNA General Medicine biology.organism_classification Molecular biology Blotting Southern chemistry Recombinant DNA biology.protein Mergus Pseudogenes |
| Zdroj: | Gene. 87:193-197 |
| ISSN: | 0378-1119 |
| Popis: | Two highly repeated nucleotide sequences (RBMI and RBMII) cloned from an EcoRI digest of DNA of the redbreasted merganser (Mergus serrator) account for approx. 5 to 10% of the DNA of M. serrator and the closely related Mergus merganser. Complete DNA digestion of seven members of the Mergini with EcoRI produces distinct, relatively species-specific patterns of a few high-Mr (greater than 1.5 kb) fragments of RBMI-like material. In such digests RBMII forms ladder-type patterns with monomers of approx. 200 bp. The sequence of a cloned 2.6-kb RBMI fragment from M. serrator contains several extended (up to 70 bp) and modified poly(dA) sequences, two open reading frames in opposite orientation to the longest poly(dA) sequence and two direct 10-bp repeats suggesting that RBMI is a rearranged retropseudogene-like element. |
| Databáze: | OpenAIRE |
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