Secretory TRAIL-Armed Natural Killer Cell–Based Therapy: In Vitro and In Vivo Colorectal Peritoneal Carcinomatosis Xenograft
| Autor: | Lisa Bailey, David L. Bartlett, Xinxin Song, Se Hoon Hong, William T. Kwon, Yong J. Lee, Yong Tae Kwon, Per H. Basse |
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| Rok vydání: | 2016 |
| Předmět: |
Cytotoxicity
Immunologic Male 0301 basic medicine Cancer Research Genetic Vectors Apoptosis Biology Immunotherapy Adoptive Article Natural killer cell Viral vector TNF-Related Apoptosis-Inducing Ligand Mice 03 medical and health sciences Interleukin 21 0302 clinical medicine Cell Line Tumor Gene Order medicine Animals Humans Cytotoxic T cell Peritoneal Neoplasms Lymphokine-activated killer cell Genetic Therapy Xenograft Model Antitumor Assays Tumor Burden Killer Cells Natural Disease Models Animal 030104 developmental biology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Immunology Cancer research Interleukin 12 Tumor necrosis factor alpha Colorectal Neoplasms |
| Zdroj: | Molecular Cancer Therapeutics. 15:1591-1601 |
| ISSN: | 1538-8514 1535-7163 |
| Popis: | Since its discovery in 1995, TNF-related apoptosis-inducing ligand (TRAIL) has sparked growing interest among oncologists due to its remarkable ability to induce apoptosis in malignant human cells, but not in most normal cells. However, one major drawback is its fast clearance rate in vivo. Thus, the development of an alternative means of delivery may increase the effectiveness of TRAIL-based therapy. In this study, we developed a secretory TRAIL-armed natural killer (NK) cell–based therapy and assessed its cytotoxic effects on colorectal cancer cells and its tumoricidal efficacy on colorectal peritoneal carcinomatosis xenograft. We generated genetically modified NK cells by transduction with a lentiviral vector consisting of a secretion signal domain, a trimerization domain, and an extracellular domain of the TRAIL gene. These NK cells secreted a glycosylated form of TRAIL fusion protein that induced apoptotic death. Intraperitoneally, but not intravenously, injected NK cells effectively accumulated at tumor sites, infiltrated tumor tissue, induced apoptosis, and delayed tumor growth. These results shed light on the therapeutic potential of genetically engineered NK cells to treat peritoneal carcinomatosis. Mol Cancer Ther; 15(7); 1591–601. ©2016 AACR. |
| Databáze: | OpenAIRE |
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