Chemosensitivity testing of fresh human leukemia cells using both a dye exclusion assay and a tetrazolium dye (MTT) assay
| Autor: | M Duke, D L Kirkpatrick, T S Goh |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Cancer Research
Human leukemia Pathology medicine.medical_specialty Cell Survival Chronic lymphocytic leukemia Tetrazolium Salts Antineoplastic Agents chemistry.chemical_compound Predictive Value of Tests In vivo Acute lymphocytic leukemia Tumor Cells Cultured medicine Humans MTT assay Coloring Agents Leukemia business.industry Hematology medicine.disease Molecular biology Thiazoles Oncology chemistry Drug Screening Assays Antitumor Formazan business Chemosensitivity assay |
| Zdroj: | Leukemia Research. 14:459-466 |
| ISSN: | 0145-2126 |
| Popis: | Chemosensitivity testing using a DEA was performed on 91 leukemia samples from 70 patients with leukemia. In 55 patients, comparison of in-vitro results with actual response of the patient was made. There was good correlation in 82% of the cases, the results being highly significant p less than 0.001. The MTT assay was also adapted for the chemosensitivity testing and the usefulness of this assay was evaluated by comparing the results to the DEA for 24 of the samples. Survival results produced by the two assays were very similar. The MTT procedure was also found to be equally effective as the DEA as a predictive assay of chemosensitivity. |
| Databáze: | OpenAIRE |
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