BROMATOMATRIC ASSAY OF GATIFLOXACIN IN PHARMACEUTICALS
| Autor: | Urdigere Rangachar Anil Kumar, Kanakapura Basavaiah, Kalsang Tharpa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Chromatography
Bromine formulations medicine.diagnostic_test Chemistry General Chemical Engineering chemistry.chemical_element lcsh:TP155-156 Hydrochloric acid Thymol blue assay bromate-bromide Absorbance chemistry.chemical_compound Indigo carmine Spectrophotometry Reagent gatifloxacin medicine Methyl orange spectrophotometry lcsh:Chemical engineering lcsh:HD9650-9663 lcsh:Chemical industries |
| Zdroj: | Chemical Industry and Chemical Engineering Quarterly, Vol 14, Iss 3, Pp 185-190 (2008) |
| ISSN: | 1451-9372 |
| Popis: | Three new, simple, and cost-effective visible spectrophotometric methods are proposed for determination of gatifloxacin (GTF) using bromate-bromide mixture, and three dyes, methyl orange, indigocarmine and thymol blue, as reagents. The methods engross the addition of a known excess of bromate-bromide mixture to GTF in hydrochloric acid medium followed by determination of residual bromine by reacting with a fixed amount of either methyl orange and measuring the absorbance at 520 nm (method A) or indigo carmine and measuring the absorbance at 610 nm (method B) or thymol blue and measuring the absorbance at 550 nm (method C) In all the methods, the amount of bromine reacted corresponds to the amount of GTF and the absorbance Is found to Increase linearly with the concentration of GTF Under the optimum conditions, GTF could be assayed in the concentration range 0.25-1.5, 0.5-6.0, and 0.5-10 mu g/mL by method A method B and method C, respectively. The apparent molar absorptivities are calculated to be 1.6 x 10(5), 4.0 x 10(4) and 3.2 x 10(4) L mol(-1) cm(-1) for the method A, method B and method C, respectively and the corresponding Sandell sensitivity values are 0.0025, 0.010 and 0.012 mu g/cm(2). The intra-day and Inter-day precision, and the accuracy of the methods were evaluated as per the current ICH guidelines. The methods were successfully applied to the determination of GTF in pharmaceutical preparations without the interference from any of the pharmaceutical adjuvants. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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