Inhibition of autophagy after perforator flap surgery increases flap survival and angiogenesis
| Autor: | Hongqiang Wu, Zhicheng Jin, Long Wang, Weiyang Gao, Jieke Wang, Shao Chen |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Vascular Endothelial Growth Factor A medicine.medical_specialty Angiogenesis Survival ratio VEGF receptors CD34 Drug Evaluation Preclinical Neovascularization Physiologic Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Autophagy Flap survival Medicine Animals Histological examination biology business.industry Adenine Graft Survival Surgery Vascular endothelial growth factor 030104 developmental biology chemistry 030220 oncology & carcinogenesis Microvessels biology.protein business Perforator Flap |
| Zdroj: | The Journal of surgical research. 231 |
| ISSN: | 1095-8673 |
| Popis: | Background The survival ratio of multiterritory perforator flap is variable. Therefore, surviving mechanisms are increasingly explored to identify novel therapeutics. The condition of the choke zone is essential for perforator flap survival. In this study, we investigated autophagy in the choke zone after flap surgery. Materials and methods The flap model involved a perforator flap with three territories that was located on the right dorsal side of a rat. A total of 36 rats were divided into six groups, including the control, 0 d postoperative (PO), 1, 3, 5, and 7 d PO groups. In addition, 72 rats were divided into three groups, including a control group, a 3-methyladenine (3-MA) group, and a rapamycin group. Skin tissue of rats was used for measuring autophagy proteins, vascular endothelial growth factor (VEGF) expression, and histological examination. On day 7 after surgery, the survival ratio of each flap was determined. Results The expression of autophagy and VEGF in the second choke zone (choke II) was increased after flap surgery. Among the three groups, the survival ratio of flaps in the 3-MA group was the highest. Furthermore, the angiogenesis level in the 3-MA group in choke II was the highest among the three groups. Conclusions Autophagy was initiated by surgery in choke II, and VEGF expression in choke II was increased after flap surgery. Inhibiting autophagy after perforator flap surgery is beneficial for flap survival and for promoting angiogenesis in choke II. |
| Databáze: | OpenAIRE |
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