The rhesus rotavirus outer capsid protein VP4 functions as a hemagglutinin and is antigenically conserved when expressed by a baculovirus recombinant
| Autor: | Erich R. Mackow, H Chan, J W Barnett, Harry B. Greenberg |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Rotavirus
medicine.drug_class viruses Genetic Vectors Immunology DNA Recombinant Hemagglutinins Viral Hemagglutinin (influenza) Insect Viruses Sf9 medicine.disease_cause Monoclonal antibody complex mixtures Microbiology Virus Capsid fluids and secretions Virology medicine Polyhedrin Animals Glycophorin Glycophorins Antigens Viral biology virus diseases Hemagglutination Inhibition Tests Macaca mulatta Precipitin Tests Molecular biology Insect Science biology.protein Receptors Virus Research Article |
| Zdroj: | Journal of Virology. 63:1661-1668 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.63.4.1661-1668.1989 |
| Popis: | Rhesus rotavirus (RRV) gene 4 was cloned into lambda bacteriophage, inserted into a polyhedrin promoter shuttle plasmid, and expressed in Sf9 cells by a recombinant baculovirus. The baculovirus-expressed VP4 protein made up approximately 5% of the Spodoptera frugiperda-infected cell protein. Monoclonal antibodies that neutralize the virus bound to the expressed VP4 polypeptide, indicating that the expressed VP4 protein was antigenically indistinguishable from viral VP4. In addition, we have determined that the baculovirus-expressed VP4 protein bound to erythrocytes and functions as the RRV hemagglutinin. The endogenous hemagglutinating activity of the VP4 protein, like the virus, was inhibited by guinea pig antirotavirus hyperimmune serum and by VP4-specific neutralizing monoclonal antibodies. The human erythrocyte protein, glycophorin, also inhibited hemagglutination by RRV or the expressed VP4 protein and appears to be the rotavirus erythrocyte receptor. The baculovirus-expressed VP4 protein was conserved functionally and antigenically in the absence of other outer or inner capsid rotavirus components and represents a logical candidate for future immunological studies. |
| Databáze: | OpenAIRE |
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