Vibrational spectroscopy of liquid biopsies for prostate cancer diagnosis
| Autor: | Moreau, Johanna, Bouzy, Pascaline, Guillard, Julien, Garnotel, Roselyne, Marchal, Aude, Gobinet, Cyril, Terryn, Christine, Thiéfin, Gérard, Medipally, Dinesh, Cullen, Daniel, Untereiner, Valérie, Sockalingum, Ganesh, Maguire, Adrian, Nguyen, Thi Nguyet Que, Bryant, Jane, Noone, Emma, Bradshaw, Shirley, Finn, Marie, Dunne, Mary, Shannon, Aoife, Armstrong, John, Meade, Aidan, Lyng, Fiona |
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| Přispěvatelé: | Biospectroscopie Translationnelle - EA 7506 (BIOSPECT), Université de Reims Champagne-Ardenne (URCA), Plateforme en Imagerie Cellulaire et Tissulaire (PICT), Université de Reims Champagne-Ardenne (URCA)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Pathology
medicine.medical_specialty principal component analysis [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] Infrared spectroscopy [SDV.CAN]Life Sciences [q-bio]/Cancer [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] Malignancy lcsh:RC254-282 03 medical and health sciences Prostate cancer 0302 clinical medicine Prostate Diagnosis [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] medicine Liquid biopsy ComputingMilieux_MISCELLANEOUS 030304 developmental biology Original Research 0303 health sciences medicine.diagnostic_test liquid biopsy business.industry Rectal examination medicine.disease prostate cancer lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens 3. Good health [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biophysics Prostate-specific antigen FTIR spectroscopy medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Raman spectroscopy [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic partial least squares discriminant analysis and classical least squares fitting analysis business [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing |
| Zdroj: | Therapeutic Advances in Medical Oncology, Vol 12 (2020) Therapeutic Advances in Medical Oncology Therapeutic Advances in Medical Oncology, SAGE Journals, 2020, 12 (18), pp.175883592091849. ⟨10.1177/1758835920918499⟩ Articles |
| ISSN: | 1758-8359 |
| Popis: | Background: Screening for prostate cancer with prostate specific antigen and digital rectal examination allows early diagnosis of prostate malignancy but has been associated with poor sensitivity and specificity. There is also a considerable risk of over-diagnosis and over-treatment, which highlights the need for better tools for diagnosis of prostate cancer. This study investigates the potential of high throughput Raman and Fourier Transform Infrared (FTIR) spectroscopy of liquid biopsies for rapid and accurate diagnosis of prostate cancer. Methods: Blood samples (plasma and lymphocytes) were obtained from healthy control subjects and prostate cancer patients. FTIR and Raman spectra were recorded from plasma samples, while Raman spectra were recorded from the lymphocytes. The acquired spectral data was analysed with various multivariate statistical methods, principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and classical least squares (CLS) fitting analysis. Results: Discrimination was observed between the infrared and Raman spectra of plasma and lymphocytes from healthy donors and prostate cancer patients using PCA. In addition, plasma and lymphocytes displayed differentiating signatures in patients exhibiting different Gleason scores. A PLS-DA model was able to discriminate these groups with sensitivity and specificity rates ranging from 90% to 99%. CLS fitting analysis identified key analytes that are involved in the development and progression of prostate cancer. Conclusions: This technology may have potential as an alternative first stage diagnostic triage for prostate cancer. This technology can be easily adaptable to many other bodily fluids and could be useful for translation of liquid biopsy-based diagnostics into the clinic. |
| Databáze: | OpenAIRE |
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