Pbp1, the yeast ortholog of human Ataxin-2, functions in the cell growth on non-fermentable carbon sources
| Autor: | Arvin Lapiz Valderrama, Tomoaki Mizuno, Yasuyuki Suda, Dang Thi Tuong Vi, Eri Matsuura, Ayaka Ito, Shiori Fujii, Kaoru Irie, Ayaka Nishihata, Kenji Irie |
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| Rok vydání: | 2021 |
| Předmět: |
Microarrays
Mutant Gene Expression Mitochondrion Biochemistry Untranslated Regions Gene Expression Regulation Fungal Energy-Producing Organelles Ataxin-2 Multidisciplinary biology Chemistry Messenger RNA Eukaryota Mitochondria Cell biology Nucleic acids Mutant Strains Bioassays and Physiological Analysis Medicine Cellular Structures and Organelles Research Article Exonuclease Saccharomyces cerevisiae Proteins 3' Utr Science Saccharomyces cerevisiae Bioenergetics Research and Analysis Methods Poly(A)-Binding Proteins Genetics Humans Gene Regulation FBP1 Cell growth Gluconeogenesis Organisms Fungi Biology and Life Sciences Promoter Cell Biology Carbon Yeast Fermentation Mutation biology.protein RNA Gene Deletion |
| Zdroj: | PLoS ONE, Vol 16, Iss 5, p e0251456 (2021) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0251456 |
| Popis: | Pbp1, the yeast ortholog of human Ataxin-2, was originally isolated as a poly(A) binding protein (Pab1)-binding protein. Pbp1 regulates the Pan2-Pan3 deadenylase complex, thereby modulating the mRNA stability and translation efficiency. However, the physiological significance of Pbp1 remains unclear since a yeast strain harboring PBP1 deletion grows similarly to wild-type strain on normal glucose-containing medium. In this study, we found that Pbp1 has a role in cell growth on the medium containing non-fermentable carbon sources. While the pbp1Δ mutant showed a similar growth compared to the wild-type cell on a normal glucose-containing medium, the pbp1Δ mutant showed a slower growth on the medium containing glycerol and lactate. Microarray analyses revealed that expressions of the genes involved in gluconeogenesis, such as PCK1 and FBP1, and of the genes involved in mitochondrial function, such as COX10 and COX11, were decreased in the pbp1Δ mutant. Pbp1 regulated the expressions of PCK1 and FBP1 via their promoters, while the expressions of COX10 and COX11 were regulated by Pbp1, not through their promoters. The decreased expressions of COX10 and COX11 in the pbp1Δ mutant were recovered by the loss of Dcp1 decapping enzyme or Xrn1 5’-3’exonuclease. Our results suggest that Pbp1 regulates the expressions of the genes involved in gluconeogenesis and mitochondrial function through multiple mechanisms. |
| Databáze: | OpenAIRE |
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