The sensitivity of an interferon-γ release assay in microbiologically confirmed pediatric tuberculosis
| Autor: | K Schopfer, Yay Chantana, Denis R. St. Laurent, Hans L. Rieder, Peter Studer, Beat Richner, Thomas Bodmer, J.F. Steinlin-Schopfer |
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| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.medical_specialty Tuberculosis Gastroenterology Sensitivity and Specificity Mycobacterium tuberculosis Interferon-gamma Interferon γ Internal medicine medicine Humans Child Whole blood biology business.industry Infant medicine.disease biology.organism_classification Pediatric tuberculosis Clinical diagnosis Child Preschool Pediatrics Perinatology and Child Health Immunology Female Disease manifestation business Cambodia Interferon-gamma Release Tests |
| Zdroj: | European journal of pediatrics. 173(3) |
| ISSN: | 1432-1076 |
| Popis: | This study aimed at determining the sensitivity of a whole blood interferon-γ release assay (IGRA) among children with microbiologically confirmed tuberculosis in a high-burden country. Children with a diagnosis of tuberculosis based on clinical and radiographic assessment were tested with an IGRA in addition to microbiologic examination of appropriate specimens for acid-fast bacilli, mycobacterial rRNA, and observation for growth of Mycobacterium tuberculosis on appropriate culture media. Of the 405 children with a clinical diagnosis of tuberculosis, 91 (22.5 %) had microbiologically confirmed tuberculosis, of whom 81 were tested with an IGRA. A positive result was obtained in 43 (sensitivity 53.1 %, 95 % confidence interval 42.3 to 63.6 %), uninfluenced by age, sex, or disease manifestation. Conclusions: The sensitivity of a whole blood interferon-γ release assay in microbiologically confirmed pediatric tuberculosis was low. An IGRA cannot, thus, be used as rule-in test, but it might be useful to rule in tuberculosis among children in whom tuberculosis is notoriously difficult to confirm microbiologically. |
| Databáze: | OpenAIRE |
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