A silicon nanomembrane platform for the visualization of immune cell trafficking across the human blood–brain barrier under flow
| Autor: | Fabien Gosselet, Adrien Mossu, Hideaki Nishihara, Britta Engelhardt, Tejas S. Khire, James L. McGrath, Maria Rosito, Federica Sallusto, Henry H. Chung, Lucie Dehouck, Isabelle Gruber, Emma Luke |
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| Přispěvatelé: | Theodor Kocher Institute, University of Bern, University of Rochester [USA], Laboratoire de la Barrière Hémato-Encéphalique (LBHE), Université d'Artois (UA), Institute for Research in Biomedicine, Theodor Kocher Institut |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
[SDV]Life Sciences [q-bio]
Cell blood–brain barrier microfluidics nanoporous silicon nitride membrane t-cell migration two-compartmental flow chamber blood-brain barrier coculture techniques endothelial cells humans immune system nanotechnology pericytes permeability silicon compounds cell movement membranes artificial models biological artificial 610 Medicine & health Blood–brain barrier Models Biological 03 medical and health sciences 0302 clinical medicine Immune system Live cell imaging medicine Neuroinflammation Human blood Membranes Artificial medicine.anatomical_structure Membrane Neurology membranes Physiological flow Biophysics 570 Life sciences biology Neurology (clinical) Cardiology and Cardiovascular Medicine 030217 neurology & neurosurgery Rapid Communication |
| Zdroj: | Journal of Cerebral Blood Flow and Metabolism Journal of Cerebral Blood Flow and Metabolism, Nature Publishing Group, 2018, 39 (3), pp.395-410. ⟨10.1177/0271678X18820584⟩ Mossu, Adrien; Rosito, Maria; Khire, Tejas; Li Chung, Hung; Nishihara, Hideaki; Gruber, Isabelle; Luke, Emma; Dehouck, Lucie; Sallusto, Federica; Gosselet, Fabien; McGrath, James L; Engelhardt, Britta (2019). A silicon nanomembrane platform for the visualization of immune cell trafficking across the human blood-brain barrier under flow. Journal of cerebral blood flow and metabolism, 39(3), pp. 395-410. Sage 10.1177/0271678X18820584 |
| ISSN: | 0271-678X 1559-7016 |
| Popis: | International audience; Here we report on the development of a breakthrough microfluidic human in vitro cerebrovascular barrier (CVB) model featuring stem cell-derived brain-like endothelial cells (BLECs) and nanoporous silicon nitride (NPN) membranes (µSiM-CVB). The nanoscale thinness of NPN membranes combined with their high permeability and optical transparency makes them an ideal scaffold for the assembly of an in vitro microfluidic model of the blood-brain barrier (BBB) featuring cellular elements of the neurovascular unit (NVU). Dual-chamber devices divided by NPN membranes yield tight barrier properties in BLECs and allow an abluminal pericyte-co-culture to be replaced with pericyte-conditioned media. With the benefit of physiological flow and superior imaging quality, the µSiM-CVB platform captures each phase of the multi-step T-cell migration across the BBB in live cell imaging. The small volume of |
| Databáze: | OpenAIRE |
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