Bioactive nanoparticle-based formulations increase survival area of perforator flaps in a rat model
| Autor: | Mihai A. Constantinescu, Ioana Lese, David Graf, Martin T. Matter, Inge K. Herrmann, Catherine Tsai, Adriano Taddeo, Radu Olariu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
CD31
Ceramics Physiology Angiogenesis Anti-Inflammatory Agents lcsh:Medicine Bioactive Glasses 02 engineering and technology Pathology and Laboratory Medicine Epithelium 0302 clinical medicine Animal Cells Immune Physiology Blood plasma Medicine and Health Sciences Nanotechnology lcsh:Science 610 Medicine & health Materials Immune Response Skin Innate Immune System Multidisciplinary Chemistry Laser Doppler velocimetry 021001 nanoscience & nanotechnology Body Fluids Blood 030220 oncology & carcinogenesis Physical Sciences Toxicity Engineering and Technology Cytokines Anatomy Cellular Types 0210 nano-technology Perfusion Research Article Biotechnology Histology Materials Science Immunology Bioengineering Blood Plasma Biomaterials Necrosis 03 medical and health sciences Signs and Symptoms Diagnostic Medicine Animals Inflammation Tissue Survival lcsh:R Biology and Life Sciences Endothelial Cells Epithelial Cells Cell Biology Blood flow Molecular Development Rats Biological Tissue Immune System Nanoparticles lcsh:Q Perforator Flap Perforator flaps Developmental Biology Biomedical engineering |
| Zdroj: | Lese, Ioana; Graf, David Alexander; Tsai, Catherine; Taddeo, Adriano; Matter, Martin Tobias; Constantinescu, Mihai Adrian; Herrmann, Inge Katrin; Olariu, Radu (2018). Bioactive nanoparticle-based formulations increase survival area of perforator flaps in a rat model. PLoS ONE, 13(11), e0207802. Public Library of Science 10.1371/journal.pone.0207802 PLoS ONE, Vol 13, Iss 11, p e0207802 (2018) PLoS ONE PLoS ONE, 13 (11) |
| ISSN: | 1932-6203 |
| DOI: | 10.7892/boris.121845 |
| Popis: | Background Distal flap necrosis is a frequent complication of perforator flaps. Advances in nanotechnology offer exciting new therapeutic approaches. Anti-inflammatory and neo-angiogenic properties of certain metal oxides within the nanoparticles, including bioglass and ceria, may promote flap survival. Here, we explore the ability of various nanoparticle formulations to increase flap survival in a rat model. Materials and methods A 9 x 3 cm dorsal flap based on the posterior thigh perforator was raised in 32 Lewis rats. They were divided in 4 groups and treated with different nanoparticle suspensions: I–saline (control), II–Bioglass, III–Bioglass/ceria and IV–Zinc-doped strontium-substituted bioglass/ceria. On post-operative day 7, planimetry and laser Doppler analysis were performed to assess flap survival and various samples were collected to investigate angiogenesis, inflammation and toxicity. Results All nanoparticle-treated groups showed a larger flap survival area as compared to the control group (69.9%), with groups IV (77,3%) and II (76%) achieving statistical significance. Blood flow measurements by laser Doppler analysis showed higher perfusion in the nanoparticle-treated flaps. Tissue analysis revealed higher number of blood vessels and increased VEGF expression in groups II and III. The cytokines CD31 and MCP-1 were decreased in groups II and IV. Conclusions Bioglass-based nanoparticles exert local anti-inflammatory and neo-angiogenic effects on the distal part of a perforator flap, increasing therefore its survival. Substitutions in the bioglass matrix and trace metal doping allow for further tuning of regenerative activity. These results showcase the potential utility of these nanoparticles in the clinical setting. PLoS ONE, 13 (11) ISSN:1932-6203 |
| Databáze: | OpenAIRE |
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