PAR-CLIP: A Method for Transcriptome-Wide Identification of RNA Binding Protein Interaction Sites
| Autor: | Markus Hafner, Charles H. Danan, Sudhir Manickavel |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Binding Sites Gene Expression Profiling Intron RNA-dependent RNA polymerase RNA High-Throughput Nucleotide Sequencing RNA-Binding Proteins RNA-binding protein Biology Non-coding RNA Molecular biology Article Cell biology 03 medical and health sciences RNA silencing 030104 developmental biology RNA editing Humans Immunoprecipitation Ribonucleosides Transcriptome Small nuclear RNA Protein Binding |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071618509 Methods in Molecular Biology ISBN: 9781493930661 |
| DOI: | 10.1007/978-1-0716-1851-6_9 |
| Popis: | During post-transcriptional gene regulation (PTGR), RNA binding proteins (RBPs) interact with all classes of RNA to control RNA maturation, stability, transport, and translation. Here, we describe Photoactivatable-Ribonucleoside-Enhanced Cross linking and Immunoprecipitation (PAR-CLIP), a transcriptome-scale method for identifying RBP binding sites on target RNAs with nucleotide-level resolution. This method is readily applicable to any protein directly contacting RNA, including RBPs that are predicted to bind in a sequence- or structure-dependent manner at discrete RNA recognition elements (RREs), and those that are thought to bind transiently, such as RNA polymerases or helicases. |
| Databáze: | OpenAIRE |
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