Origin and Consequences of Chromosomal Inversions in thevirilisGroup ofDrosophila
| Autor: | Jorge Vieira, Nico Posnien, Micael Reis, Rodrigo Lata, Cristina P. Vieira |
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| Přispěvatelé: | Instituto de Investigação e Inovação em Saúde |
| Rok vydání: | 2018 |
| Předmět: |
Drosophila
Chromosom 0301 basic medicine Transposable element ectopic recombination Genome Insect Genome 03 medical and health sciences chromosomal inversions Drosophila americana Drosophila novamexicana Genetics Melanogaster Animals Ectopic recombination Drosophila (subgenus) Gene Ecology Evolution Behavior and Systematics Whole Genome Sequencing biology myr biology.organism_classification 030104 developmental biology Evolutionary biology Chromosome Inversion Genomic Structural Variation Female Drosophila melanogaster Research Article |
| Zdroj: | Genome Biology and Evolution Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| ISSN: | 1759-6653 |
| Popis: | In Drosophila, large variations in rearrangement rate have been reported among different lineages and among Muller’s elements. Nevertheless, the mechanisms that are involved in the generation of inversions, their increase in frequency, as well as their impact on the genome are not completely understood. This is in part due to the lack of comparative studies on species distantly related to Drosophila melanogaster. Therefore, we sequenced and assembled the genomes of two species of the virilis phylad (Drosophila novamexicana [15010-1031.00] and Drosophila americana [SF12]), which are diverging from D. melanogaster for more than 40 Myr. Based on these data, we identified the precise location of six novel inversion breakpoints. A molecular characterization provided clear evidence that DAIBAM (a miniature inverted–repeat transposable element) was involved in the generation of eight out of the nine inversions identified. In contrast to what has been previously reported for D. melanogaster and close relatives, ectopic recombination is thus the prevalent mechanism of generating inversions in species of the virilis phylad. Using pool-sequencing data for three populations of D. americana, we also show that common polymorphic inversions create a high degree of genetic differentiation between populations for chromosomes X, 4, and 5 over large physical distances. We did not find statistically significant differences in expression levels between D. americana (SF12) and D. novamexicana (15010-1031.00) strains for the three genes surveyed (CG9588, Fig 4, and fab1) flanking three inversion breakpoints. This article is a result of the project Norte-01-0145-FEDER-000008—Porto Neurosciences and Neurologic Disease Research Initiative at I3S, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). N.P. and M.R. are funded by the Emmy Noether Programme of the Deutsche Forschungsgemeinschaft (Grant Number: PO 1648/3-1 to N.P.). We would like to thank the Transcriptome Analysis Lab (TAL) (University Medical Center Göttingen, UMG) in Göttingen for the Illumina sequencing. |
| Databáze: | OpenAIRE |
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