Live attenuated Edwardsiella ictaluri vaccines enhance the protective innate immune responses of channel catfish B cells
| Autor: | Wei Tan, Adef O. Kordon, Kara Majors, Lesya M. Pinchuk, Attila Karsi, Hossam Abdelhamed, Safak Kalindamar |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Phagocytosis Immunology Apoptosis Biology Adaptive Immunity Protective Agents Vaccines Attenuated Microbiology 03 medical and health sciences Fish Diseases 0302 clinical medicine Sepsis Animals Edwardsiella ictaluri Opsonin Catfishes B-Lymphocytes Innate immune system Attenuated vaccine Vaccination Immunity Innate Antibody opsonization 030104 developmental biology Bacterial Vaccines Host-Pathogen Interactions 030215 immunology Developmental Biology Catfish |
| Zdroj: | Developmental and comparative immunology. 109 |
| ISSN: | 1879-0089 |
| Popis: | Edwardsiella ictaluri causes enteric septicemia of catfish. Our group developed two E. ictaluri live attenuated vaccines (LAVs). However, their effects on the innate functions of catfish B cells are still unexplored. We evaluated phagocytosis and killing of wild-type (WT) E. ictaluri opsonized with sera from vaccinated fish and the survival of B cells exposed to E. ictaluri strains. We assessed phagocytosis of the opsonized WT at 30 °C and 4 °C. B cells killed the internalized E. ictaluri opsonized with sera from vaccinated fish with LAVs more efficiently than other groups at 30 °C. However, catfish B cells were unable to destroy E. ictaluri at 4 °C. Furthermore, E. ictaluri opsonized with serum from fish exposed to WT induce apoptosis and decreased live B cells numbers. Results indicate that opsonization of E. ictaluri with sera from vaccinated fish enhanced phagocytosis and killing activity in B cells and inhibited apoptotic changes in the infected B cells. |
| Databáze: | OpenAIRE |
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