Identification of Filovirus Entry Inhibitors Targeting the Endosomal Receptor NPC1 Binding Site
| Autor: | Robert A. Davey, Nicholas Y. Palermo, J. J. Patten, Leslie Estrada, Shi Hua Xiang, Colton Thompson, Leah Liu Wang, Manu Anantpadma |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cell Survival 030106 microbiology Biology Antiviral Agents Article Marburg virus 03 medical and health sciences Inhibitory Concentration 50 Viral envelope Viral Envelope Proteins Niemann-Pick C1 Protein Virology Drug Discovery Filoviridae Infections Humans Cytotoxicity Pharmacology chemistry.chemical_classification Binding Sites Host Microbial Interactions RNA Virus Internalization Ebolavirus In vitro Molecular Docking Simulation Hemorrhagic Fevers 030104 developmental biology chemistry Marburgvirus Receptors Virus NPC1 Glycoprotein HeLa Cells Protein Binding |
| Zdroj: | Antiviral Res |
| Popis: | Filoviruses, mainly consisting of Ebola viruses (EBOV) and Marburg viruses (MARV), are enveloped negative-strand RNA viruses which can infect humans to cause severe hemorrhagic fevers and outbreaks with high mortality rates. The filovirus infection is mediated by the interaction of viral envelope glycoprotein (GP) and the human endosomal receptor Niemann-Pick C1 (NPC1). Blocking this interaction will prevent the infection. Therefore, we utilized an In silico screening approach to conduct virtual compound screening against the NPC1 receptor-binding site (RBS). Twenty-six top-hit compounds were purchased and evaluated by in vitro cell based inhibition assays against pseudotyped or replication-competent filoviruses. Two classes (A and U) of compounds were identified to have potent inhibitory activity against both Ebola and Marburg viruses. The IC50 values are in the lower level of micromolar concentrations. One compound (compd-A) was found to have a sub-micromolar IC50 value (0.86 μM) against pseudotyped Marburg virus. The cytotoxicity assay (MTT) indicates that compd-A has a moderate cytotoxicity level but the compd-U has much less toxicity and the CC50 value was about 100 μM. Structure-activity relationship (SAR) study has found some analogs of compd-A and -U have reduced the toxicity and enhanced the inhibitory activity. In conclusion, this work has identified several qualified lead-compounds for further drug development against filovirus infection. |
| Databáze: | OpenAIRE |
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