Synthesis and immunodetection of 6-O-methyl-phosphoramidyl-α-D-galactose: a Campylobacter jejuni antigenic determinant
| Autor: | Patricia Guerry, Zuchao Ma, Mario A. Monteiro, Yuening Jiao, Cheryl P. Ewing |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
01 natural sciences
Biochemistry Campylobacter jejuni Epitope Analytical Chemistry Microbiology 03 medical and health sciences chemistry.chemical_compound Conjugate vaccine Carbohydrate Conformation 030304 developmental biology Antiserum 0303 health sciences Vaccines Conjugate biology 010405 organic chemistry Chemistry Immunogenicity Organic Chemistry Polysaccharides Bacterial Phosphoramidate Galactosides General Medicine biology.organism_classification 0104 chemical sciences 3. Good health Galactose Phosphoramides Conjugate |
| Zdroj: | Carbohydrate research. 418 |
| ISSN: | 1873-426X |
| Popis: | Campylobacter jejuni is a leading cause of traveler's diarrhea. Previously, we have shown that a C. jejuni capsule polysaccharide (CPS) conjugate vaccine can fully prevent C.jejuni diarrhea in non-human primates. C.jejuni CPSs are decorated with non-stoichiometric amounts of O-methyl phosphoramidate (MeOPN) units that are key serospecific markers. In the case of C.jejuni serotype complex HS23/36, the MeOPN are at positions 2 and 6 of the CPS galactose (Gal). We describe here the synthesis of the p-methoxyphenyl glycoside of MeOPN→6-α-D-Galp, and its immunodetection by antisera raised by C.jejuni CPS conjugates with MeOPN at primary positions. The synthetic approach in this work served as the foundation for a similar MeOPN→6-Gal construct used in a conjugate vaccine, whose synthesis, immunogenicity and efficacy will be described elsewhere. |
| Databáze: | OpenAIRE |
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